ABSTRACT A survey of entomopathogenic nematodes (EPN) was conducted in Morocco during 2015 and 2016 in three regions with varying topography, climate and vegetation (Meknès-El Hajeb, Ifrane and Tafilalet). EPN were isolated from 14 of 169 soil samples (8%). Molecular and morphological techniques were used for species determination. Nine samples contained heterorhabditids and five samples contained steinernematids. Eight heterorhabditids were identified as Heterorhabditis bacteriophora Poinar 1976, two steinernematids as Steinernema feltiae (Filipjev, 1934), three as belonging to the S. feltiae-group, and one as an unidentified isolate (Heterorhabditis sp.) which gives dark burgundy colour for G. mellonella cadavers. These EPNs were collected mainly from agricultural soils rather than natural habitats, with potential adaptation to specific geography, climate and soil conditions (texture, pH and organic matter content), including six EPN isolates from heavy soils. These data demonstrate that EPN are present in Moroccan soils but at relatively low frequency, so opportunity exists to augment these populations to gain biological control benefits.

中文翻译：

---

昆虫病原线虫（Steinernematidae 和 Heterorhabditidae）在摩洛哥的发生和分布

摘要 2015 年和 2016 年，摩洛哥在三个地形、气候和植被各不相同的地区（Meknès-El Hajeb、Ifrane 和 Tafilalet）进行了昆虫病原线虫 (EPN) 调查。EPN 从 169 个土壤样品中的 14 个 (8%) 中分离出来。分子和形态学技术用于物种确定。九个样品含有异线虫，五个样品含有丝线虫。8 种异线虫被鉴定为 Heterorhabditis bacteriophora Poinar，1976 年被鉴定为 Heterorhabditis bacteriophora Poinar，两种 steinernematids 被鉴定为 Steinernema Feeliae (Filipjev, 1934)，三种属于 S. Feeliae 组，一种被鉴定为一种未鉴定的分离物 (Heterorhabditis sp.)，其颜色为暗色。 . 蜜蜡尸体。这些 EPN 主要来自农业土壤而不是自然栖息地，具有对特定地理、气候和土壤条件（质地、pH 值和有机质含量）的潜在适应能力，包括从重土壤中分离出的六种 EPN。这些数据表明 EPN 存在于摩洛哥土壤中，但频率相对较低，因此存在增加这些种群以获得生物防治效益的机会。