Abstract

Ultrasonic-assisted extraction methods have been applied in many fields in recent decades. In this study, chlorogenic acid was isolated from capillary artemisia ultrasonically using the designed poly (natural deep eutectic solvents-ethylene glycol dimethacrylate) cellulose. The actual quantity of chlorogenic acid extracted was 62.83 mg g⁻¹dry powder using the following optimal conditions: raw material to liquid ratio of 1:40 g mL⁻¹, extraction temperature of 80 °C, ultrasonic power of 160 W, and extraction time of 40 min. The limit of detection for chlorogenic acid in capillary artemisia was 0.14 µg mL⁻¹, and the intraday and interday precision values as the relative standard deviation (n = 6) after spiking with 5, 100, and 500 µg mL⁻¹ were both less than 4.21%. This paper reports an effective method that provides facile operation, is low cost, and has excellent selectivity for chlorogenic acid in capillary artemisia.

摘要

近几十年来，超声辅助提取方法已在许多领域得到应用。在这项研究中，使用设计的聚（天然深共熔溶剂-乙二醇二甲基丙烯酸酯）纤维素从毛细蒿中超声分离绿原酸。在以下最佳条件下，提取的绿原酸的实际量为62.83 mg g ^-1干粉：原料与液体的比例为1:40 g mL ^-1，提取温度为80°C，超声功率为160 W，提取时间为40分钟。毛细管蒿中绿原酸的检测限为0.14 µg mL ^-1，日内和日间精密度值作为相对标准偏差（n = 6）加标5、100和500 µg mL ^-1后，两者均小于4.21％。本文报道了一种有效的方法，该方法操作简便，成本低廉，并且对毛细血管蒿中的绿原酸具有优异的选择性。