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The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX‐20
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-09-30 , DOI: 10.1111/1751-7915.13658 Wenchao Chen 1, 2, 3, 4 , Meng Wang 1 , Yangmin Gong 1, 2, 3, 4 , Qianchun Deng 1, 2, 3, 4 , Mingming Zheng 1, 2, 3, 4 , Shouwen Chen 5 , Xia Wan 1, 2, 3, 4 , Chen Yang 1, 2, 3, 4 , Fenghong Huang 1, 2, 3, 4
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-09-30 , DOI: 10.1111/1751-7915.13658 Wenchao Chen 1, 2, 3, 4 , Meng Wang 1 , Yangmin Gong 1, 2, 3, 4 , Qianchun Deng 1, 2, 3, 4 , Mingming Zheng 1, 2, 3, 4 , Shouwen Chen 5 , Xia Wan 1, 2, 3, 4 , Chen Yang 1, 2, 3, 4 , Fenghong Huang 1, 2, 3, 4
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Fungal pretreatment is the most common strategy for improving the conversion of rapeseed meal (RSM) into value‐added microbial products. It was demonstrated that Bacillus amyloliquefaciens CX‐20 could directly use RSM as the sole source of all nutrients except the carbon source for iturin A fermentation with high productivity. However, whether fungal pretreatment has an impact on iturin A production is still unknown. In this study, the effects of fungal pretreatment and direct bio‐utilization of RSM for iturin A fermentation were comparatively analysed through screening suitable fungal species, and evaluating the relationships between iturin A production and the composition of solid fermented RSM and liquid hydrolysates. Three main unconventional adverse effects were identified. (1) Solid‐state fermentation by fungi resulted in a decrease of the total nitrogen for B. amyloliquefaciens CX‐20 growth and metabolism, which caused nitrogen waste from RSM. (2) The released free ammonium nitrogen in liquid hydrolysates by fungal pretreatment led to the reduction of iturin A. (3) The insoluble precipitates of hydrolysates, which were mostly ignored and wasted in previous studies, were found to have beneficial effects on producing iturin A. In conclusion, our study verifies the unconventional adverse effects of fungal pretreatment on iturin A production by B. amyloliquefaciens CX‐20 compared with direct bio‐utilization of RSM.
中文翻译：
真菌预处理对解淀粉芽孢杆菌CX-20发酵iturin A的非常规不利影响
真菌预处理是提高菜籽粕（RSM）转化为增值微生物产品的最常用策略。证明了解淀粉芽孢杆菌CX-20可以直接将RSM用作所有营养素的唯一来源，但伊图林A发酵的碳源除外，可实现高生产率。但是，真菌预处理是否会影响iturin A的产生仍是未知的。在这项研究中，通过筛选合适的真菌种类并评估iturin A产量与固态发酵RSM和液体水解产物组成之间的关系，比较了真菌预处理和RSM对iturin A发酵的直接生物利用的影响。确定了三个主要的非常规不良反应。（1）真菌的固态发酵导致解淀粉芽孢杆菌的总氮减少CX-20的生长和代谢导致RSM浪费氮。（2）通过真菌预处理在液体水解产物中释放的游离铵态氮导致iturin A的减少。（3）水解产物的不溶性沉淀物在以前的研究中通常被忽略和浪费，被发现对生产iturin具有有益的作用。答：总而言之，我们的研究验证了真菌预处理对解淀粉芽孢杆菌CX-20产生的Iturin A产生的非常规不利影响，这与RSM的直接生物利用相比。
更新日期：2020-09-30
中文翻译：
真菌预处理对解淀粉芽孢杆菌CX-20发酵iturin A的非常规不利影响
真菌预处理是提高菜籽粕（RSM）转化为增值微生物产品的最常用策略。证明了解淀粉芽孢杆菌CX-20可以直接将RSM用作所有营养素的唯一来源，但伊图林A发酵的碳源除外，可实现高生产率。但是，真菌预处理是否会影响iturin A的产生仍是未知的。在这项研究中，通过筛选合适的真菌种类并评估iturin A产量与固态发酵RSM和液体水解产物组成之间的关系，比较了真菌预处理和RSM对iturin A发酵的直接生物利用的影响。确定了三个主要的非常规不良反应。（1）真菌的固态发酵导致解淀粉芽孢杆菌的总氮减少CX-20的生长和代谢导致RSM浪费氮。（2）通过真菌预处理在液体水解产物中释放的游离铵态氮导致iturin A的减少。（3）水解产物的不溶性沉淀物在以前的研究中通常被忽略和浪费，被发现对生产iturin具有有益的作用。答：总而言之，我们的研究验证了真菌预处理对解淀粉芽孢杆菌CX-20产生的Iturin A产生的非常规不利影响，这与RSM的直接生物利用相比。
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