The combination of highly sensitive techniques such as electrochemiluminescence (ECL) with nanotechnology sparked new analytical applications, in particular for immunoassay‐based detection systems. In this context, nanomaterials, particularly dye‐doped silica nanoparticles (DDSNPs) are of high interest, since they can offer several advantages in terms of sensitivity and performance. In this work we synthesized two sets of monodispersed and biotinylated [Ru(bpy)₃]²⁺‐doped silica nanoparticles, named bio‐Triton@RuNP and bio‐Igepal@RuNP, obtained following the reverse microemulsion method using two different types of nonionic surfactants. Controlling the synthetic procedures, we were able to obtain nanoparticles (NPs) offering highly intense signal, using tri‐n‐propylamine (TPrA) as coreactant, with bio‐Triton@RuNps being more efficient than bio‐Igepal@RuNP.

中文翻译：

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染料掺杂的二氧化硅纳米粒子增强了基于ECL的免疫分析性能

电化学发光（ECL）等高灵敏度技术与纳米技术的结合，激发了新的分析应用，特别是基于免疫测定的检测系统。在这种情况下，纳米材料尤其是染料掺杂的二氧化硅纳米颗粒（DDSNP）备受关注，因为它们可以在灵敏度和性能方面提供多个优势。在这项工作中，我们合成了两组单分散和生物素化的[Ru（bpy）₃ ] ²⁺掺杂的二氧化硅纳米粒子，分别称为bio-Triton @ RuNP和bio-Igepal @ RuNP，这是通过反相微乳液法使用两种不同类型的非离子表面活性剂获得的表面活性剂。控制合成过程中，我们能够获得纳米颗粒（NP）提供高强度的信号，使用三ñ丙胺（TPrA）作为共反应剂，bio-Triton @ RuNps比bio-Igepal @ RuNP更有效。