Abstract Alginate encapsulation is amongst the most studied probiotic encapsulation formulations in the literature, preferred for its low toxicity, low cost and ability to protect entrapped probiotics against acidic pH. Despite extensive academic studies, there has been little commercial translation of alginate-encapsulated probiotic formulations. In this study, we hypothesized that factors affecting freeze-drying survivability of alginate-encapsulated probiotics could have limited its translation potential. Factors including: 1) encapsulant material, 2) cryoprotectant, 3) cross-linker, 4) Concentration of crosslinker, and 5) other Group II divalent cations cross-linkers were investigated, using two model probiotics – Lactobacillus rhamnosus GG (LGG) and Escherichia coli Nissle 1917 (ECN). Divalent cations, which are used to cross-link alginates, were found to have antagonistic effects on the freeze-drying survivability of LGG and ECN. To mitigate the deleterious effects of divalent cations, the addition of sucrose was found to be useful, possibly due to its membrane stabilization mechanism.

中文翻译：

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二价阳离子对封装在交联海藻酸盐中的冻干益生菌的生存能力具有拮抗作用

摘要 海藻酸盐胶囊是文献中研究最多的益生菌胶囊制剂之一，因其低毒性、低成本和保护包裹的益生菌免受酸性 pH 的影响而受到青睐。尽管进行了广泛的学术研究，但几乎没有对藻酸盐封装的益生菌制剂进行商业翻译。在这项研究中，我们假设影响海藻酸盐包封益生菌冷冻干燥存活率的因素可能限制了其转化潜力。因素包括：1) 封装材料，2) 冷冻保护剂，3) 交联剂，4) 交联剂的浓度，以及 5) 使用两种模型益生菌 - 鼠李糖乳杆菌 GG (LGG) 和其他 II 组二价阳离子交联剂进行了研究大肠杆菌 Nissle 1917 (ECN)。二价阳离子，用于交联藻酸盐，发现对 LGG 和 ECN 的冻干存活率具有拮抗作用。为了减轻二价阳离子的有害影响，发现添加蔗糖是有用的，这可能是由于其膜稳定机制。