With an increasing interest for the use of triploids in abalone aquaculture, it is crucial to understand their physiological responses to environmental stress, particularly such as heat-stress and hypoxia, which are significant factors that cause adverse effects on the efficiency and capacity of farming practice in abalone production. However, nothing is known about gene expression of triploid abalone to modulate physiological responses under different environmental stresses. Transcriptomic response to the acute heat-stress and hypoxia were explored in hepatopancreas of diploid and triploid Pacific abalone (Haliotis discus hannai) juveniles. A total of 316 million clean reads were de novo assembled into 271,039 contigs, of which a transcriptome with 209,974 non-redundant transcripts was produced. Using generalized fold change (GFOLD) algorithm with a cut-off │GFOLD value│ > 4, we identified differentially expressed transcripts (DETs) from diploid and triploid abalone in responses to acute heat-stress and hypoxia treatments, respectively. Comparative analysis of the identified DETs revealed alteration of transcript expression profile, level, and process in triploid abalone compared to their diploid siblings. Thus, our study will provide not only comprehensive insight into understanding of the transcriptional regulation to environmental stresses in triploid abalone but a framework for efficient management of triploid abalone aquaculture.

随着人们对三倍体在鲍鱼养殖中的应用越来越感兴趣，了解它们对环境压力的生理反应至关重要，尤其是热应激和缺氧等，这些都是对养殖效率和能力产生不利影响的重要因素在鲍鱼生产中。然而，关于三倍体鲍鱼在不同环境压力下调节生理反应的基因表达尚不清楚。在二倍体和三倍体太平洋鲍鱼 ( Haliotis discus hannai ) 幼鱼的肝胰腺中探索了对急性热应激和缺氧的转录组反应。总共 3.16 亿次干净读取是从头开始的组装成 271,039 个重叠群，其中产生了具有 209,974 个非冗余转录本的转录组。使用具有截止值│GFOLD 值│> 4 的广义倍数变化 (GFOLD) 算法，我们确定了来自二倍体和三倍体鲍鱼分别响应急性热应激和缺氧处理的差异表达转录本 (DET)。已鉴定的 DET 的比较分析显示，与其二倍体鲍鱼相比，三倍体鲍鱼的转录表达谱、水平和过程发生了改变。因此，我们的研究不仅将为理解三倍体鲍鱼环境胁迫的转录调控提供全面的见解，而且将为三倍体鲍鱼水产养殖的有效管理提供一个框架。