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Oxidative stress and genotoxicity in Rhinella arenarum (Anura: Bufonidae) tadpoles after acute exposure to Ni-Al nanoceramics
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.etap.2020.103508
Gabriela Svartz , Carolina Aronzon , Soledad Pérez Catán , Sonia Soloneski , Cristina Pérez Coll

The employ of nanomaterials (NMs) has exponentially grown due to the large number of technological advances in industrial, pharmaceutical and medical areas. That is the case of alumina (Al) nanoparticles which are extensively employed as support in heterogeneous catalysis processes. However, these NMs can cause great toxicity because of their ubiquitous properties, such as extremely small size and high specific surface area. So, it is required to assess the potential deleterious effects of these NMs on living organisms. In the present study, we analyze the oxidative stress and genotoxic potential of a nanoceramic catalyst Ni/<gamma>-Al2O3 (NC) and the NMs involved in their synthesis, <gamma>-Al2O3 support (SPC) and NiO/<gamma>-Al2O3 precursor (PC) on Rhinella arenarum larvae. Biomarkers of oxidative stress and genotoxic damage were measured in tadpoles exposed to 5 and 25 mg/L of each NMs for 96 h. The results indicated an inhibition of catalase activity in tadpoles exposed to both concentrations of PC and to 25 mg/L of SPC and NC. Moreover, both exposure concentrations of PC and NC significantly inhibited superoxide dismutase activity. Exposure to the three NMs caused inhibition of glutathione S-transferase activity, but there were no significant variations in reduced glutathione levels. Oxidative stress damage (lipid peroxidation) was observed in tadpoles treated with 25 mg/L PC, while the other treatments did not produce alterations. The MNs frequency significantly increased in larvae exposed to 25 mg/L PC indicating irreversible genotoxic damage. The results show that these NMs exert genotoxic effects and antioxidant defense system disruption in R. arenarum larvae.



中文翻译:

急性暴露于Ni-Al纳米陶瓷后,黑毛tAnhuiBufonidaepole的氧化应激和遗传毒性

由于工业,制药和医疗领域的大量技术进步,纳米材料(NMs)的使用呈指数增长。氧化铝(Al)纳米颗粒就是这种情况,它被广泛用作非均相催化过程中的载体。但是,这些NM由于无处不在的特性(例如极小的尺寸和高的比表面积)而可能引起巨大的毒性。因此,需要评估这些NM对生物的潜在有害作用。在本研究中,我们分析了氧化应激和纳米陶瓷催化剂的潜在遗传毒性的Ni / <伽玛> -Al 2 ö 3(NC)和参与其合成的NMS,<伽玛> -Al 2 ö 3支持(SPC)和NiO / <γ> -Al莱茵兰竞技场上的2 O 3前体(PC)幼虫。在暴露于5和25 mg / L的每个NMs 96小时的t中测量了氧化应激和遗传毒性破坏的生物标志物。结果表明,暴露于PC浓度和25 mg / L SPC和NC的t中的过氧化氢酶活性均受到抑制。此外,PC和NC的暴露浓度均显着抑制超氧化物歧化酶活性。暴露于三个NM引起谷胱甘肽S-转移酶活性的抑制,但是减少的谷胱甘肽水平没有明显变化。在用25 mg / L PC处理的t中观察到氧化应激损伤(脂质过氧化),而其他处理未产生变化。在暴露于25 mg / L PC的幼虫中,MNs的频率显着增加,表明不可逆的遗传毒性破坏。R. arenarum幼虫。

更新日期:2020-10-11
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