The disparity between the endogenous antioxidants concentration and free radicals in spermatozoa results in reactive oxygen species (ROS) generation. In this prospect, epigallocatechin-3-gallate (EGCG) preserves vigorous antioxidant features. Current study explored the influence of EGCG in a cryo-diluent media on microscopic parameters, oxidative stress parameters, and fertility potential of buffalo spermatozoa during cryopreservation. Concisely, collected semen from three donor bulls for four times were then evaluated for volume, motility, concentrations and dilution in a cryo-diluent media with different concentrations of EGCG (EGCG-0 = control; EGCG-50 = 50 μM, EGCG-100 = 100 μM, EGCG-200 = 200 μM, and EGCG-300 = 300 μM) at 37 °C, cooled to 4 °C in 2 h, equilibrated for 4 h at 4 °C, and cryopreserved. At post-thawing, Computer-Assisted Sperm motion Analysis motilities (total and progressive, %) and rapid velocity (%) plasma membrane functionality, supravital plasma membrane integrity, and mitochondrial potential (%) found higher (P < 0.05) in EGCG-200 and EGCG-300 than control, whereas average-path, straight-line and curved-linear velocities (μm/sec) and acrosome integrity (%) were higher in EGCG-300 than control. Further, comet length (μm) and tail length (μm), LPO (lipid peroxidation, μM/mL) and apoptosis-like changes (%) in spermatozoa were significantly decreased in EGCG-300 than control. Sperm antioxidant enzymes activities (glutathione peroxidase, U/mL and superoxide dismutase, U/mL) were increased with EGCG-300 than control. Moreover, EGCG-300 addition in a cryo-diluent media improves the fertility potential (%) of buffalo spermatozoa. In a nutshell, the inclusion of EGCG-300 in a cryo-diluent media enhances post-thaw microscopic parameters, and fertility potential, whereas decreases oxidative stress parameters in buffalo spermatozoa.

中文翻译：

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在冷冻稀释介质中添加表没食子儿茶素-3-没食子酸酯 (EGCG) 作为抗氧化剂可改善微观参数和生育潜力，并减轻水牛精子的氧化应激参数

精子中内源性抗氧化剂浓度和自由基之间的差异导致活性氧（ROS）的产生。在这一前景中，表没食子儿茶素-3-没食子酸酯 (EGCG) 保留了强大的抗氧化特性。目前的研究探讨了冷冻稀释介质中 EGCG 对冷冻保存期间水牛精子的微观参数、氧化应激参数和生育潜力的影响。简而言之，从三只供体公牛收集的精液四次，然后在具有不同浓度 EGCG（EGCG-0 = 对照；EGCG-50 = 50 μM，EGCG-100 = 100 μM、EGCG-200 = 200 μM 和 EGCG-300 = 300 μM) 在 37 °C 下，在 2 小时内冷却至 4 °C，在 4 °C 下平衡 4 小时，并冷冻保存。解冻后，在 EGCG-200 和 EGCG-300 中，计算机辅助精子运动分析（总和渐进式，%）和快速速度（%）质膜功能、超生质膜完整性和线粒体电位（%）更高（P < 0.05） EGCG-300 的平均路径、直线和曲线速度 (μm/sec) 和顶体完整性 (%) 高于对照。此外，EGCG-300 中的彗星长度（μm）和尾长（μm）、LPO（脂质过氧化，μM/mL）和精子中的细胞凋亡样变化（％）显着低于对照。与对照组相比，EGCG-300 的精子抗氧化酶活性（谷胱甘肽过氧化物酶，U/mL 和超氧化物歧化酶，U/mL）增加。此外，在冷冻稀释介质中添加 EGCG-300 可提高水牛精子的生育潜力 (%)。简而言之，