Pulsed electric field (PEF) treatment can be used to increase intracellular small molecule concentrations in bacteria, which can lead to enhanced robustness of these cells during further processing. In this study we investigated the effects of the PEF treatment temperature and the presence of 8% (v/v) ethanol in the PEF medium on cell survival, membrane fluidity and intracellular trehalose concentrations of Lactobacillus plantarum WCFS1. A moderate PEF treatment temperature of 21 °C resulted in a high cell survival combined with higher intracellular trehalose concentrations compared to a treatment at 10 and 35 °C. Interestingly, highest intracellular trehalose concentrations were observed upon supplementing the PEF medium with 8% ethanol, which resulted in more than a doubling in intracellular trehalose concentrations, while culture survival was retained. Overall, this study shows that treatment temperature and PEF medium optimization are important directions for improving molecule uptake upon PEF processing.

脉冲电场（PEF）处理可用于增加细菌中细胞内小分子的浓度，这可能导致这些细胞在进一步加工过程中的坚固性增强。在这项研究中，我们研究了PEF处理温度和PEF培养基中8％（v / v）乙醇的存在对植物乳杆菌细胞存活，膜流动性和细胞内海藻糖浓度的影响。WCFS1。与在10和35°C的处理相比，中等的PEF处理温度为21°C导致较高的细胞存活率和较高的细胞内海藻糖浓度。有趣的是，在向PEF培养基中添加8％乙醇后，观察到了最高的细胞内海藻糖浓度，这导致细胞内海藻糖浓度增加了一倍以上，同时保留了培养物的存活率。总的来说，这项研究表明处理温度和PEF培养基的优化是改善PEF加工过程中分子吸收的重要方向。