Abstract

This paper presents a new technique of hydrocole (bacteria, blue−green algae, phytoplankton, zooplankton, sponges, fish oil and seal fat) fatty acids (FA) methyl esters determination by gas-liquid chromatography. The polyunsaturated fatty acids (PUFA) contents in these objects were different. The distinguishing features of the technique are the next ones: efficiency, lipid extraction recovery ≥94% using the sonication during 10–15 min without sample drying, high PUFAs yield (≥97%) by reduction of oxidation up to 40% during methylation; exhaustive extraction of fatty acid methyl esters (FAMEs) including PUFA; a new approach to obtain of methyl esters of free fatty acids except using toxic reagents; accuracy of FAMEs quantification including ω-3-, ω-6-, ω-9-FA using the di-n-decyl ether as an internal standard and accuracy of measurements using “T14165QC Fish oil” standard sample (first). The technique is approbated for Baikal hydrocole FA determination from 20 to 2000 μg in the sample and might be used for scientific and applied tasks. The interlaboratory precision of FAME determination of phytoplankton and bacteria is ≤10%.

中文翻译：

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气液色谱法测定不同含量的多不饱和脂肪酸的油酸中的游离和酯化脂肪酸

摘要

本文介绍了一种通过气液色谱法测定脂肪酸（FA）甲基酯的新技术（细菌，蓝藻，浮游植物，浮游动物，海绵，鱼油和海豹脂肪）。这些对象中的多不饱和脂肪酸（PUFA）含量不同。这项技术的显着特点是：效率高，在不干燥样品的情况下在10-15分钟内使用超声波进行脂质提取，脂类提取回收率≥94％，在甲基化过程中通过氧化还原高达40％的高PUFA收率（≥97％）；彻底萃取包括PUFA的脂肪酸甲酯（FAME）；除使用有毒试剂外，获得游离脂肪酸甲酯的新方法；使用di- n进行ω-3-，ω-6-，ω-9-FA的FAME定量的准确性-癸醚作为内标，并使用“ T14165QC鱼油”标准样品进行测量（第一个）。该技术适用于测定样品中20至2000μg的贝加尔湖水合物FA，可用于科学和应用任务。FAME测定浮游植物和细菌的实验室间精密度≤10％。