Laccases (EC 1.10.3.2) are multi-copper oxidases that can degrade several xenobiotics, including textile dyes. Present study investigated the nature of laccase isoforms induced by 2,6-dimethylaniline in Cyathus bulleri cultivated on basal salt medium. Two isoforms, LacI and LacII were identified and purified by a combination of ultrafiltration and ion-exchange chromatography. The MS spectrum of the two proteins displayed a number of non-identical and identical molecular peaks (m/z), and, the latter were mapped to protein originating from the previously reported Laccase (Lcc) 1 gene. The LacI isoform exhibited higher catalytic efficiency (Kcat/Km) towards 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), 2,6-dimethoxyphenol, guaiacol and pyrogallol and was tolerant to high levels of chloride ions and resistant to EDTA. Higher decolorization of several dyes such as Direct Scarlet B (67%), Reactive Brilliant blue-R (96%), Direct Orange 34 (50%) and Reactive Red198 (95%) by the LacI isoform makes it a good candidate for degradation of synthetic dyes. The decolorization of Direct Orange 34 by laccases is being reported for the first time. Many of the properties exhibited by this isoform make it a good candidate for large scale production and applications for use in the dyeing industry.

中文翻译：

---

Cyathus Bulleri 基础培养基上漆酶异构体的多样性及其在纺织染料和废水脱色/解毒中的作用

漆酶 (EC 1.10.3.2) 是多铜氧化酶，可以降解多种异生物质，包括纺织染料。本研究调查了在基础盐培养基上培养的 Cyathus Bulleri 中由 2,6-二甲基苯胺诱导的漆酶异构体的性质。两种异构体 LacI 和 LacII 通过超滤和离子交换色谱的组合进行鉴定和纯化。这两种蛋白质的 MS 谱显示了许多不同和相同的分子峰 (m/z)，并且后者被映射到源自先前报道的漆酶 (Lcc) 1 基因的蛋白质。LacI 异构体对 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)、2,6-dimethylphenol、愈创木酚和连苯三酚表现出更高的催化效率（Kcat/Km），并且能够耐受高水平的氯离子并耐 EDTA。LacI 同种型对直接猩红 B (67%)、活性亮蓝-R (96%)、直接橙 34 (50%) 和活性红 198 (95%) 等几种染料的更高脱色率使其成为降解的良好候选者合成染料。漆酶对 Direct Orange 34 的脱色是首次报道。这种异构体表现出的许多特性使其成为大规模生产和应用于染色工业的理想选择。