Irradiation is a quarantine treatment option for stored product pests. In the present studies, we try to investigate the effect of low gamma irradiation doses in the range of 20–300 Gy on the different developmental stages of Rhyzopertha dominica in wheat grains. It was found that no adults emerged after irradiation of eggs, larvae, and pupae at 140, 240, and 260 Gy, respectively. Treatment of adult weevils at the radiation dose of 180 Gy resulted in 71.33% mortality after 14 days post-treatment and there was no adult emergence in the F1 generation. When the adult emergence of the F1 generation was used for the measuring effective irradiation dose and phytosanitary efficacy, 280 Gy was required for quarantine security of wheat weevils. The comet assay serves to verify the DNA damage induced by the phytosanitary irradiation dose, 280 Gy on the R. dominica, and wheat grains. DNA damage was measured as the percentage of comet tail DNA. A significant linear increase in DNA damage was observed in the irradiated adult stage of R. dominica but there is no DNA damage in the wheat grains. Also, the wheat grains viability (in terms of wheat germination) was not affected by the dose level, 280 Gy. Thus, an irradiation dose of 280 Gy is recommended as an effective phytosanitary irradiation dose for quarantine security to control lesser grain borer.

中文翻译：

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用彗星试验评价辐照检疫剂量-诱导的多米尼克根霉的DNA损伤及其对小麦籽粒的影响

辐照是储存产品害虫的检疫处理选择。在目前的研究中，我们试图研究 20-300 Gy 范围内的低伽马辐射剂量对小麦籽粒中多米尼克根霉不同发育阶段的影响。发现卵、幼虫和蛹分别在 140、240 和 260 Gy 照射后没有成虫出现。以 180 Gy 的辐射剂量处理成年象鼻虫，在治疗后 14 天后死亡率为 71.33%，并且 F1 代没有成虫出现。以F1代成虫羽化为测定有效辐照剂量和植物检疫效力时，小麦象鼻虫检疫安全需要280 Gy。彗星试验用于验证由植物检疫辐射剂量 280 Gy 对 R 引起的 DNA 损伤。多米尼克和小麦谷物。DNA 损伤测量为彗尾 DNA 的百分比。在辐照后的多米尼加小麦成虫阶段观察到 DNA 损伤显着线性增加，但小麦籽粒中没有 DNA 损伤。此外，小麦籽粒活力（就小麦发芽而言）不受 280 Gy 剂量水平的影响。因此，推荐 280 Gy 的辐照剂量作为检疫安全的有效植物检疫辐照剂量，以控制较小的螟虫。