Granulosa cells (GCs) and theca cells (TCs) are the main components of follicles, and the interactions between GCs and TCs play a significant role in steroidogenesis, follicular growth, and atresia. However, the effects of GCs in the form of conditioned medium on steroidogenesis in buffalo TCs remain unclear. In the present study, the impacts of GC-conditioned medium (GCCM) on androgen synthesis in buffalo TCs were examined. The results showed that GCCM collected at 48 h promoted both the expression levels of androgen synthesis-related genes (CYP11A1, CYP17A1, 3β-HSD, and Star) and the secretion levels of testosterone in TCs. The treatment time of 48 h in GCCM improved both the expression levels of androgen synthesis-related genes (CYP11A1, CYP17A1, 3β-HSD, and Star) and the secretion levels of testosterone in TCs. Furthermore, GCCM that was collected at 48 h and applied to TCs for 48 h (48 h and 48 h) promoted the sensitivity of buffalo TCs to LH. This study indicated that GCCM (48 h and 48 h) enhanced the steroidogenic competence of TCs mainly through facilitating the responsiveness of TCs to LH in buffalo. This study provides a basis for further exploration of interactions between GCs and TCs for steroidogenesis in the ovary.

颗粒细胞 (GCs) 和卵泡细胞 (TCs) 是卵泡的主要成分，GCs 和 TCs 之间的相互作用在类固醇生成、卵泡生长和闭锁中起重要作用。然而，条件培养基形式的 GC 对水牛 TC 中类固醇生成的影响仍不清楚。在本研究中，研究了 GC 条件培养基 (GCCM) 对水牛 TC 雄激素合成的影响。结果表明在48小时收集的GCCM促进雄激素的合成相关的基因（两者的表达水平CYP11A1，CYP17A1，3β-HSD，和星）和睾酮的TCS中的分泌水平。GCCM 48 h 的处理时间提高了雄激素合成相关基因的表达水平（CYP11A1，CYP17A1，3β-HSD，和星）和睾酮的热带气旋的分泌水平。此外，在 48 小时收集并应用于 TC 48 小时（48 小时和 48 小时）的 GCCM 提高了水牛 TC 对 LH 的敏感性。该研究表明，GCCM（48 小时和 48 小时）主要通过促进 TC 对水牛 LH 的反应来增强 TC 的类固醇生成能力。该研究为进一步探索 GC 和 TC 之间相互作用对卵巢类固醇生成提供了基础。