The use of biofuels obtained from lignocellulosic materials is a technology that offers great potential for their production in the future. Agave bagasse is one of the most common wastes produced in Mexico through the Syrup, Tequila, and Mezcal industry. In particular, Tequila and Mezcal are one of the largest alcoholic beverages in the country and thousands of tons of this waste are produced annually. Therefore, agave bagasse is a promising alternative in Mexico to obtain second-generation bioethanol (2G). In this work, the use of enzymatic hydrolysates obtained by an alkaline pretreatment and enzymatic hydrolysis optimized using Agave tequilana Weber var. azul bagasse (AT) and Agave karwinskii bagasse (AK) was evaluated for ethanol production. For this, the optimization design of alkaline pretreatment and enzymatic hydrolysis was carried out using a Box-Behnken design. Both hydrolysates were fermented using Saccharomyces cerevisiae ITV01 yeast. The results showed that the best lignin reduction in the alkaline pretreatment was 77.6 and 84.29% utilizing AT and AK, respectively. Furthermore, a glucose concentration of 100.06–98.08 and 64.03–60.0 g/L was found in the enzymatic hydrolysis (AT and AK, respectively), reaching in the fermentation step a yield of 0.47 and 0.46 g/g, volumetric productivity of 1.97 and 1.14 g/L/h and a fermentation efficiency of 93.1 and 90.4% using AT and AK, respectively. These results are interesting to use agave bagasse for ethanol production in Mexico as an alternative to give added value and take advantage of its availability.

从木质纤维素材料获得的生物燃料的使用是一项在未来生产中具有巨大潜力的技术。龙舌兰蔗渣是在墨西哥通过糖浆，龙舌兰酒和梅斯卡尔工业生产的最常见的废物之一。特别是，龙舌兰酒和梅斯卡尔酒是该国最大的酒精饮料之一，每年产生数千吨这种废物。因此，龙舌兰蔗渣是墨西哥获得第二代生物乙醇（2G）的有前途的替代品。在这项工作中，使用了通过碱预处理获得的酶解产物和使用龙舌兰龙舌兰Weber var优化的酶解过程。阿祖尔蔗渣（AT）和龙舌兰karwinskii评估蔗渣（AK）的乙醇产量。为此，使用Box-Behnken设计进行了碱预处理和酶水解的优化设计。两种水解产物均用酿酒酵母发酵ITV01酵母。结果表明，利用AT和AK进行碱预处理的最佳木质素还原率分别为77.6％和84.29％。此外，在酶促水解（分别为AT和AK）中发现葡萄糖浓度分别为100.06-98.08和64.03-60.0 g / L，在发酵步骤中分别达到0.47和0.46 g / g的产量，1.97的体积生产率以及使用AT和AK分别为1.14 g / L / h和93.1％和90.4％的发酵效率。将龙舌兰蔗渣用于墨西哥的乙醇生产，以提供附加值并利用其可用性，这是有趣的结果。