Co-occurrence of two devastating foliar-fungal diseases of peanut, viz., late leaf spot (LLS), and rust may cause heavy yield loss besides adversely affecting the quality of kernel and fodder. This study reports the mapping of seven novel stress-related candidate EST-SSRs in a region having major QTLs for LLS and rust diseases using an F₂ mapping population (GJG17 × GPBD4) consisting of 328 individuals. The parental polymorphism using 1311 SSRs revealed 84 SSRs (6.4%) as polymorphic and of these 70 SSRs could be mapped on 14 linkage groups (LG). QTL analysis has identified a common QTL (LLS_QTL1/Rust_QTL) for LLS and rust diseases in the map interval of 1.41 cM on A03 chromosome, explaining 47.45% and 70.52% phenotypic variations, respectively. Another major QTL for LLS (LLS_QTL1), explaining a 29.06% phenotypic variation was also found on LG_A03. A major rust QTL has been validated which was found harboring R-gene and resistance-related genes having a role in inducing hypersensitive response (HR). Further, 23 linked SSRs including seven novel EST-SSRs were also validated in 177 diverse Indian groundnut genotypes. Twelve genotypes resistant to both LLS and rust were found carrying the common (rust and LLS) QTL region, LLS QTL region, and surrounding regions. These identified and validated candidate EST-SSR markers would be of great use for the peanut breeding groups working for the improvement of foliar-fungal disease resistance.

花生的两种破坏性叶面真菌病害，即晚叶斑病（LLS）和锈病同时发生，除了对籽粒和饲料的质量产生不利影响外，还可能导致严重的产量损失。本研究报告了使用由 328 个个体组成的F ₂作图群体 (GJG17 × GPBD4)在具有 LLS 和锈病主要 QTL 的区域中绘制 7 个新型胁迫相关候选 EST-SSR 的图谱。使用 1311 个 SSR 的亲本多态性显示 84 个 SSR (6.4%) 是多态的，这 70 个 SSR 中可以定位到 14 个连锁群 (LG)。QTL分析确定了一个常见的QTL（LLS _QTL1 /Rust _QTL) 对于 A03 染色体上 1.41 cM 图区间的 LLS 和锈病，分别解释了 47.45% 和 70.52% 的表型变异。LLS 的另一个主要 QTL (LLS _QTL1)，解释了在 LG_A03 上也发现了 29.06% 的表型变异。一个主要的锈病 QTL 已经得到验证，它被发现含有 R 基因和抗性相关基因，这些基因在诱导过敏反应 (HR) 中起作用。此外，还在 177 个不同的印度花生基因型中验证了 23 个相关的 SSR，包括 7 个新的 EST-SSR。发现对 LLS 和锈病具有抗性的 12 种基因型带有共同（锈病和 LLS）QTL 区域、LLS QTL 区域和周围区域。这些经鉴定和验证的候选 EST-SSR 标记对于致力于提高叶真菌抗病性的花生育种小组将非常有用。