The main objective of the present investigation included a comparative physicochemical, phytochemical along with chromatographic evaluation of Withania somnifera (L.) Dunal roots collected from various Indian climatic zones. The study includes macroscopical, microscopical, physicochemical, phytochemical and chromatographic evaluation of W. somnifera roots collected from different climatic zones (desert, plains, coastal and plateau) of India. Further, chromatographic evaluations were carried out taking Withaferine-A and Withanoloid-A as biomarkers with the help of HPTLC and HPLC analysis. The physicochemical parameters evaluated were found to be within the prescribed limits of the WHO, while the phytochemical analysis showed the higher quantities of phytoconstituents obtained from desert followed by coastal regions. The chromatographic analysis revealed the presence of maximum number of phytoconstituents in sample from coastal and desert region. Further, the quantification of Withaferine-A and Withanoloid-A was carried out using HPTLC and HPLC. The results confirmed higher quantity of both the biomarkers in samples collected from desert followed by coastal region. Thus, the study may be helpful in understanding the role of climatic zones in relation to the variability in phytochemical composition of medicinal plants such as W. somnifera. This will help in selecting the better quality of medicinal plant for preparation of herbal formulations with best therapeutic value that will serve the society.

中文翻译：

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不同气候区对Withaniasomnifera（L.）Dunal理化特性的影响

本研究的主要目的包括比较物理化学，植物化学以及色谱法对从印度不同气候区收集的Withania somnifera（L.）Dunal根进行色谱分析。该研究包括对W. somnifera的宏观，微观，理化，植物化学和色谱评估。从印度不同气候区（沙漠，平原，沿海和高原）收集的根。此外，借助于HPTLC和HPLC分析，以Withaferine-A和Withanoloidoid-A作为生物标志物进行了色谱评估。发现所评估的理化参数在世界卫生组织规定的范围内，而植物化学分析表明，从沙漠获得的植物成分数量更高，其次是沿海地区。色谱分析表明，沿海和沙漠地区样品中存在最多的植物成分。此外，使用HPTLC和HPLC进行Withaferine-A和Withanoloidoid-A的定量。结果证实，从沙漠和沿海地区采集的样品中两种生物标志物的含量较高。从而，W. somnifera。这将有助于选择更好质量的药用植物来制备具有最佳治疗价值的草药配方，从而为社会服务。