Multi-drug resistance is one of the biggest nightmares in the field of healthcare today. Adding on to this, some bacteria like Staphylococcus aureus and Pseudomonas aeruginosa have the ability to form biofilms. These essentially are large colonies of bacteria that are held together by polysaccharides and other biomolecules which in turn facilitate in their adherence to solid substrate – both natural and synthetic. This further creates a life-threatening implication leading to nosocomial infections like pneumonia, Urinary tract infections (UTI), etc. increasing the co-morbidities and mortality of critically-ill patients. The combination of antimicrobial resistance, ability to form biofilms and threat of nosocomial infections calls for a need to investigate newer, safer alternatives.

Plant based medicaments have been used for centuries and they are a great alternative to synthetic drugs. In the present study, ethanolic extracts of Hemidesmus indicus was evaluated against clinically-important multi-drug resistant organisms. Percentage biofilm inhibition of plant extracts of Hemidesmus indicus by crystal violet assay method. Triplicate analysis was done and data obtained was statistically interpreted using Microsoft Excel. Alcoholic extracts of Hemidesmus indicus exhibited significant biofilm inhibitory activity against the common bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Further, isolation of the chief active constituent responsible for Anti-biofilm activity is in process.

中文翻译：

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棉铃虫乙醇提取物的细菌生物膜抑制活性

多药耐药性是当今医疗领域最大的噩梦之一。除此之外，某些细菌如金黄色葡萄球菌和铜绿假单胞菌具有形成生物膜的能力。这些基本上是细菌的大菌落，它们由多糖和其他生物分子结合在一起，进而促进它们与固体和天然基质的粘附。这进一步造成了威胁生命的影响，导致了诸如肺炎，泌尿道感染（UTI）等医院感染，从而增加了重症患者的合并症和死亡率。抗菌素耐药性，形成生物膜的能力和医院感染的威胁相结合，需要研究更新，更安全的替代方法。

基于植物的药物已经使用了多个世纪，它们是合成药物的绝佳替代品。在本研究中，对印度梧桐的乙醇提取物针对临床上重要的耐多药生物进行了评估。结晶紫测定法抑制印度He草提取物生物膜的百分比。进行三重分析，并使用Microsoft Excel对获得的数据进行统计解释。棉铃虫的乙醇提取物对常见细菌大肠杆菌，铜绿假单胞菌，金黄色葡萄球菌和枯草芽孢杆菌具有明显的生物膜抑制活性。。此外，正在分离负责抗生物膜活性的主要活性成分。