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In vivo efficiency of Bacillus sp. isolated from biofloc system on growth, haematological, immunological and antioxidant status of genetically improved farmed tilapia (GIFT)
Indian Journal of Experimental Biology ( IF 0.6 ) Pub Date : 2020-09-30
M Menaga, S Felix, M Charulatha, A Gopalakannan, C Mohanasundari, Satyanarayana Boda

In aquaculture, Biofloc technology is emerging as one of the successful sustainable technologies to increase production. It is known to possess several immunostimulatory compounds exhibiting possible probiotic effect in culture. Here, we explored the in vivo efficiency of Bacillus infantis (T1), B. subtilis (T2), Exiguobacterium profundum (T3) and B. megaterium (T4) isolated from biofloc systems for improving the growth and immune performance of genetically improved farmed tilapia (GIFT). Animals (10+0.08 g) were stocked at a density of 100 per m-3 in 500 L FRP tanks for 42 days in triplicate. All the four probiotics (OD =1) were mixed with basal diet in treatments and feed without probiotic maintained as control. A significant difference (P <0.05) in weight gain, specific growth rate and FCR were observed between treatments and control with 100% survival. Serum albumin, globulin, protein, total blood count, glucose, myeloperoxidase activity and SOD were significantly different (P <0.05) between treatments and control. T4 and T2 showed better immunological and anti-oxidant ability when compared to other strains. Results from principal component analysis demonstrated that B. megaterium and B. subtilis can be the promising probiotic bacteria isolated from biofloc systems exhibiting multiple benefits with improved growth and health of the culture animals.

中文翻译:

芽孢杆菌的体内效率。从生物絮凝系统中分离得到的基因改良养殖罗非鱼的生长,血液学,免疫学和抗氧化状态

在水产养殖中,Biofloc技术正在成为增加产量的成功可持续技术之一。已知具有几种在培养中表现出可能的益生菌作用的免疫刺激化合物。在这里,我们探讨了从生物絮凝系统中分离的婴儿芽孢杆菌(T1),枯草芽孢杆菌(T2),深层根茎杆菌(T3)和巨大芽孢杆菌(T4)的体内效率,以改善转基因罗非鱼的生长和免疫性能(礼品)。以每米-3的密度100饲养动物(10 + 0.08 g)在500升玻璃钢储罐中一式三份,共42天。在治疗中将所有四种益生菌(OD = 1)与基础饮食混合,饲料中未添加益生菌作为对照。在治疗和100%存活率的对照之间观察到体重增加,比生长速率和FCR有显着差异(P < 0.05)。治疗与对照组之间的血清白蛋白,球蛋白,蛋白质,总血球计数,葡萄糖,髓过氧化物酶活性和SOD显着不同(P <0.05)。与其他菌株相比,T4和T2表现出更好的免疫和抗氧化能力。主成分分析的结果表明,巨大芽孢杆菌枯草芽孢杆菌 可以是从生物絮凝系统中分离出来的有前途的益生菌,具有多种益处,可以改善培养动物的生长和健康。
更新日期:2020-09-30
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