Loss of erythrocyte membrane deformability is one of the most crucial factors in developing complications associated with Type II diabetes. The observed loss of erythrocyte membrane deformability could be related to structural changes in the membrane. In this context, here, we have made an attempt at gaining a better insight (quantitative as well as qualitative) into the protein and lipid contents in erythrocyte membranes and their interrelationships in Type II diabetes. Age matched control (n=12) and Type II diabetic subjects (n=22) were selected for this study. Morphological characteristics were studied by atomic force microscopy (AFM). AFM study confirmed remarkable alterations in morphology of the diabetic erythrocytes. In diabetic erythrocytes following changes were noted: (i) Significant increase in membrane as well as cytosolic proteins with a marginal increase in phospholipids content; (ii) The membrane total lipids:protein, phospholipids:protein, cholesterol:protein and phospholipids:cholesterol (mole:mole) ratios decreased significantly; (iii). A reproducible decrease in docosahexaenoic acid (DHA) and Omega-3 index with increase in Omega-6:Omega-3 ratio in membrane fatty acids; and (iv) The SDS-PAGE analysis indicated that all membrane proteins increased in almost equal proportion leading to increased membrane protein content. The observed compositional and stochiometric changes in lipids, proteins and their ratios may underlie morphological alterations and loss of deformability.

中文翻译：

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II型糖尿病患者红细胞膜的成分变化

红细胞膜变形能力的丧失是发展与II型糖尿病相关的并发症的最关键因素之一。观察到的红细胞膜变形能力的丧失可能与膜的结构变化有关。在这种情况下，我们尝试对红细胞膜中的蛋白质和脂质含量及其在II型糖尿病中的相互关系获得更好的见解（定量和定性）。选择年龄匹配的对照组（n = 12）和II型糖尿病受试者（n = 22）进行这项研究。通过原子力显微镜（AFM）研究了形态特征。原子力显微镜研究证实了糖尿病红细胞形态的显着改变。在糖尿病红细胞中，注意到以下变化：（i）膜和胞浆蛋白显着增加，磷脂含量略有增加；（ii）膜总脂质：蛋白质，磷脂：蛋白质，胆固醇：蛋白质和磷脂：胆固醇（摩尔：摩尔）的比率显着降低；（iii）。随着膜脂肪酸中Omega-6：Omega-3比例的增加，二十二碳六烯酸（DHA）和Omega-3指数可再现降低；（iv）SDS-PAGE分析表明所有膜蛋白以几乎相等的比例增加，导致膜蛋白含量增加。观察到的脂质，蛋白质及其比率的组成和化学计量变化可能是形态学改变和变形能力丧失的基础。蛋白质和磷脂：胆固醇（摩尔：摩尔）的比例明显降低；（iii）。随着膜脂肪酸中Omega-6：Omega-3比例的增加，二十二碳六烯酸（DHA）和Omega-3指数可再现降低；（iv）SDS-PAGE分析表明所有膜蛋白以几乎相等的比例增加，导致膜蛋白含量增加。观察到的脂质，蛋白质及其比率的组成和化学计量变化可能是形态学改变和变形能力丧失的基础。蛋白质和磷脂：胆固醇（摩尔：摩尔）的比例明显降低；（iii）。随着膜脂肪酸中Omega-6：Omega-3比例的增加，二十二碳六烯酸（DHA）和Omega-3指数可再现降低；（iv）SDS-PAGE分析表明所有膜蛋白以几乎相等的比例增加，导致膜蛋白含量增加。观察到的脂质，蛋白质及其比率的组成和化学计量变化可能是形态学改变和变形能力丧失的基础。（iv）SDS-PAGE分析表明所有膜蛋白以几乎相等的比例增加，导致膜蛋白含量增加。观察到的脂质，蛋白质及其比率的组成和化学计量变化可能是形态学改变和变形能力丧失的基础。（iv）SDS-PAGE分析表明所有膜蛋白以几乎相等的比例增加，导致膜蛋白含量增加。观察到的脂质，蛋白质及其比率的组成和化学计量变化可能是形态学改变和变形能力丧失的基础。