Laser irradiation therapy on cancer cells is a promising alternative in providing a non-invasive treatment of breast cancer that has a possibility to inhibit cancerous cells selectively without damaging surrounding healthy tissues. This present study aimed to evaluate the effect of the low-level green laser of 532 nm wavelength with various laser power and irradiation time on MCF-7 cancer cell lines. In this work, the MCF-7 cells were seeded to a rate of fifty thousand cells/well in 96-well plate and incubated for 24 h. The cells were then irradiated with the green laser at different power from 0.002 to 0.1 W at 60 s, 540 s, and 900 s duration. The cell viability of the cells was measured by using Alamar Blue assay. From result, the laser irradiation on the cells was able to produce 25-40% inhibition of cell proliferation whereby the untreated cells exhibited a 93% cell viability. It was revealed that high power with longer exposure time increased cell bio-inhibition. Thus, this work using low-power green laser irradiation on cells demonstrated a significant effect on cells and was also demonstrated a promising non-invasive approach that can be used alone or in combination with several other therapies in cancer treatment.

中文翻译：

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低水平绿色激光照射对人乳腺癌细胞系的体外毒性

在提供无创治疗乳腺癌的方法上，对癌细胞进行激光照射疗法是一种有前途的选择，该方法有可能选择性抑制癌细胞而不会损害周围的健康组织。本研究旨在评估具有各种激光功率和照射时间的532 nm波长的低级绿色激光对MCF-7癌细胞系的影响。在这项工作中，将MCF-7细胞以每孔五万个细胞的速度接种到96孔板中，并孵育24小时。然后在60 s，540 s和900 s的持续时间内，用绿色激光以0.002至0.1 W的不同功率照射细胞。通过使用Alamar Blue测定法测量细胞的细胞生存力。从结果来看 在细胞上进行激光照射能够产生25-40％的细胞增殖抑制作用，从而使未处理的细胞表现出93％的细胞活力。揭示了高功率和更长的暴露时间增加了细胞的生物抑制。因此，这项在细胞上使用低功率绿色激光照射的工作证明了对细胞的显着效果，并且还证明了一种有前途的非侵入性方法，可以单独使用或与癌症治疗中的其他几种疗法结合使用。