This study has been performed to isolate a potential strain able to release the prolific amylase under non-aqueous conditions to meet the current demand in industries to substitute the amylase produced in aqueous media. A bacterial strain that produces organic solvent-stable amylase in the media containing 15% benzene was isolated from the soil. The recovered strain was identified to be Bacillus amyloliquefaciens AMY02by 16S rRNA sequencing. Under sub-merged fermentation, the optimized amylase release by this strain was found with the condition having starch (carbon source), pH 7.0, the temperature at 30°C for 48 h (incubation time). This optimized condition promoted the amylase production to be 2.04-fold higher than the culture was kept under standard condition with the basic media composition. Further, the stability of the enzyme in the presence of 20% organic solvents was assessed by incubating for 2 weeks. The enzyme was found to be active and stable in the presence of benzene, chloroform, o-xylene, and toluene. The higher organic solvent stability of this amylase production by B. amyloliquefaciens under sub-merged fermentation can be an alternative catalyst in non-aqueous media for industrial applications.

中文翻译：

---

淹没发酵条件下稳定芽孢杆菌AMY02释放增强的耐有机溶剂淀粉酶

已经进行了这项研究以分离出能够在非水条件下释放多淀粉酶的潜在菌株，以满足工业上当前的需求，以替代在水性介质中生产的淀粉酶。从土壤中分离出了在含有15％苯的培养基中产生有机溶剂稳定淀粉酶的细菌菌株。回收的菌株被鉴定为解淀粉芽孢杆菌AMY02通过16S rRNA测序。在深层发酵下，发现该菌株在具有淀粉（碳源），pH 7.0，温度在30°C的温度下48小时（孵育时间）的条件下释放出最佳的淀粉酶。这种优化的条件将淀粉酶的产量提高了2.04倍，而在标准条件下使用基本培养基组成的情况下，其培养量却保持不变。此外，通过温育2周来评估在20％有机溶剂存在下酶的稳定性。发现该酶在苯，氯仿，邻二甲苯和甲苯的存在下具有活性和稳定性。淹没式发酵下解淀粉芽孢杆菌产生的淀粉酶具有较高的有机溶剂稳定性，可以作为工业应用中非水介质中的替代催化剂。