This study examines whether the glucose regulating enzymes mediate hypoglycaemic effect of diallyl disulphide (DADS) since the biochemical mechanisms by which the latter regulates hepatic glucose-metabolizing enzymes remain unknown. Hepatic hexokinase, glucose-6-P-D and pyruvate kinase are the important glucose metabolising enzymes that control blood glucose homeostasis and considered to be potential targets for antidiabetic drugs. DADS is an important phytoconstituent of garlic (Allium sativum Linn.) which has been reported to possess hypoglycaemic effect. Diabetes was induced in rats by alloxan and the diabetic rats were given DADS for 30 days and the effect was compared with the standard hypoglycaemic drug metformin. The levels of blood glucose and insulin were measured using spectrophotometer and by ELISA method respectively. Activities of hepatic hexokinase, glucose-6-PD, and pyruvate kinase enzymes in hepatic tissues were measured in DADS and metformin treated diabetic rats. DADS significantly reduced the level of blood glucose and simultaneously augmented those of insulin, pyruvate kinase, hexokinase and glucose-6-PD enzyme activities almost similar to metformin. The hypoglycaemic effect of this compound may be explained, in part, by its inhibition of these enzyme activities and improved hepatic glucose utilization. This observation offers scope for new therapeutic approach in treating diabetes particularly in insulin- resistant cases.

中文翻译：

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二烯丙基二硫化物对糖尿病大鼠肝糖调节酶的影响

这项研究检查了葡萄糖调节酶是否介导二烯丙基二硫化物（DADS）的降血糖作用，因为后者调节肝脏葡萄糖代谢酶的生化机制仍然未知。肝己糖激酶，葡萄糖-6-PD和丙酮酸激酶是控制血糖动态平衡的重要葡萄糖代谢酶，被认为是抗糖尿病药物的潜在靶标。DADS是大蒜的重要植物成分（大蒜）Linn。），据报道具有降血糖作用。四氧嘧啶在大鼠中诱发糖尿病，给予糖尿病大鼠DADS 30天，并将其与标准降血糖药二甲双胍进行比较。用分光光度计和ELISA法分别测定血糖和胰岛素水平。在DADS和二甲双胍治疗的糖尿病大鼠中测量了肝组织中肝己糖激酶，葡萄糖6-PD和丙酮酸激酶的活性。DADS显着降低了血糖水平，同时增加了胰岛素，丙酮酸激酶，己糖激酶和葡萄糖-6-PD酶的活性，几乎与二甲双胍相似。该化合物的降血糖作用可以部分地通过其对这些酶活性的抑制和改善的肝葡萄糖利用来解释。