Photodynamic therapy works with a photosensitizer that is stimulated when exposed to a light source of a specific wavelength and produces a form of oxygen that can be used in cancer treatments. In this study, we investigated the effect of laser on apoptosis on breast cancer cell lines (MDA-MB-231) treated with verteporfin in cell culture media. Verteporfin added MDA-MB-231 cells were incubated without light for 24 hours after applying laser light at a wavelength of 695 nm at an intensity of 50 J/cm2 at various times. Anti-proliferative effects were evaluated by immunoreactivity of anti-Bcl-2 and anti-Bax antibodies by immunocytochemical staining. When anti-Bax/Anti-Bcl-2 ratio are compared, the ratio of 1.5 in the control group cells decreases in short-term laser applications, while it approaches normal values in the 7^th min after long-term laser application and reaches a very high value in the 9^th min. Therefore, our results suggest that verteporfin-mediated PDT may be a potential combined therapy strategy against breast carcinoma by increasing apoptosis.

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维替泊芬介导的光动力疗法对乳腺癌细胞的作用

光动力疗法与光敏剂协同工作，当光敏剂暴露于特定波长的光源时会受到刺激，并产生一种可用于癌症治疗的氧气。在这项研究中，我们研究了激光对Verteporfin在细胞培养基中处理的乳腺癌细胞系（MDA-MB-231）凋亡的影响。加入Verteporfin的MDA-MB-231细胞在不同时间以50 J / cm2的强度施加695 nm波长的激光后，不光照孵育24小时。通过抗Bcl-2和抗Bax抗体的免疫反应性通过免疫细胞化学染色评估抗增殖作用。比较anti-Bax / Anti-Bcl-2比率，在短期激光应用中，对照组细胞的比率为1.5，而在第7^次时接近正常值。分钟长期激光应用之后并到达在9非常高的值^日分钟。因此，我们的结果表明，维替泊芬介导的PDT可能是通过增加细胞凋亡来对抗乳腺癌的潜在联合治疗策略。