The present study aims to investigate, immunohistochemically, the role of the adaptive immune response in cardiac arrest/resuscitation-induced ischemia–reperfusion renal injury (IRI), namely to assess the presence of lymphocytes in renal tissue samples and the connection between the extent of the damage and the concentration of the lymphocytes by comparing the kidneys of non resuscitated swine with the kidneys of resuscitated swine. Twenty four swine underwent cardiac arrest (CA) via a pacemaker wire. After 7 min, without any intervention, Cardiopulmonary Resuscitation, CPR, was commenced. Five min after CPR was commenced advanced life-support, ALS. Animals were divided into resuscitated animals and non resuscitated animals. Tissue samples obtained from the two groups for immunohistological study aiming to detect T-cells, B-cells and plasma cells using CD3 + , CD20 + , and CD138 + antibodies. There seems to be a strong concentration of T lymphocytes in the kidney tissues after ischemia of both non-resuscitated and resuscitated swine. B lymphocytes, also, appear to have infiltrated the ischemic kidneys of both animal groups; nevertheless, the contribution of T lymphocytes to the induction of injury remains greater. There is no strong evidence of correlation between the plasma cells and the damage. The adaptive immune response seems to have a strong association with kidney injury and acute tubular necrosis after cardiac arrest/ resuscitation-induced ischemia–reperfusion. However, the extent to which the adaptive immune cells are involved in the induction of renal injury remains uncertain and there are many questions about the mechanism of function of these cells, the answers of which require further studies.

中文翻译：

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心脏骤停复苏诱导缺血再灌注肾损伤中的适应性免疫反应

本研究旨在通过免疫组织化学研究适应性免疫反应在心脏骤停/复苏诱导的缺血再灌注肾损伤 (IRI) 中的作用，即评估肾组织样本中淋巴细胞的存在与程度之间的联系。通过比较未复苏的猪的肾脏和复苏的猪的肾脏来评估淋巴细胞的损伤和浓度。24 头猪通过起搏器导线进行了心脏骤停 (CA)。7分钟后，在没有任何干预的情况下，开始了心肺复苏术，CPR。开始 CPR 后 5 分钟进行高级生命支持，ALS。将动物分为复苏动物和未复苏动物。从两组获得的组织样本用于免疫组织学研究，旨在检测 T 细胞，使用 CD3 + 、CD20 + 和 CD138 + 抗体的 B 细胞和浆细胞。在未复苏和复苏的猪缺血后，似乎在肾组织中存在高浓度的 T 淋巴细胞。B 淋巴细胞似乎也浸润了两组动物的缺血性肾脏。尽管如此，T淋巴细胞对诱导损伤的贡献仍然更大。没有强有力的证据表明浆细胞与损伤之间存在相关性。适应性免疫反应似乎与心脏骤停/复苏诱导的缺血再灌注后的肾损伤和急性肾小管坏死密切相关。然而，适应性免疫细胞在多大程度上参与肾损伤的诱导仍不确定，关于这些细胞的功能机制存在许多问题，