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Draft genome of Meyerozyma guilliermondii strain vka1: a yeast strain with composting potential
Journal of Genetic Engineering and Biotechnology ( IF 3.6 ) Pub Date : 2020-09-29 , DOI: 10.1186/s43141-020-00074-2
Ravisankar Valsalan , Deepu Mathew

Meyerozyma guilliermondii is a yeast which could be isolated from a variety of environments. The vka1 strain isolated and purified from the organic compost was found to have composting potential. To better understand the genes assisting the composting potential in this yeast, whole genome sequencing and sequence annotation were performed. The genome of M. guilliermondii vka1 strain was sequenced using a hybrid approach, on Illumina Hiseq-2500 platform at 100× coverage followed by Nanopore platform at 20× coverage. The de novo assembly using dual-fold approach had given draft genome of 10.8 Mb size. The genome was found to contain 5385 genes. The annotation of the genes was performed, and the enzymes identified to have roles in the degradation of macromolecules are discussed in relation to its composting potential. Annotation of the genome assembly of the related strains had revealed the unique biodegradation related genes in this strain. Phylogenetic analysis using the rDNA region has confirmed the position of this strain in the Ascomycota family. Raw reads are made public, and the genome wide proteome profile is presented to facilitate further studies on this organism. Meyerozyma guilliermondii vka1 strain was sequenced through hybrid approach and the reads were de novo assembled. Draft genome size and the number of genes in the strain were assessed and discussed in relation to the related strains. Scientific insights into the composting potential of this strain are also presented in relation to the unique genes identified in this strain.

中文翻译:

Meyerozyma guilliermondii菌株vka1的基因组草图:具有堆肥潜力的酵母菌株

Meererozyma guilliermondii是一种可以从多种环境中分离的酵母。从有机堆肥中分离纯化的vka1菌株具有堆肥潜力。为了更好地理解辅助该酵母中堆肥潜力的基因,进行了全基因组测序和序列注释。使用杂交方法,在Illumina Hiseq-2500平台上以100倍覆盖率对居里氏疟原虫vka1菌株的基因组进行测序,然后在20倍覆盖率的纳米孔平台上测序。使用双重折叠方法从头组装得到的基因组草图草案为10.8 Mb。发现该基因组包含5385个基因。进行了基因注释,并讨论了在大分子降解中具有作用的酶。相关菌株基因组组装的注释揭示了该菌株中独特的生物降解相关基因。使用rDNA区域的系统发育分析已确认该菌株在子囊菌家族中的位置。公开阅读原始资料,并提供全基因组蛋白质组图谱,以促进对该生物的进一步研究。通过杂交方法对古细菌耶尔森菌vka1菌株进行测序,并从头开始重新装配读段。评估了基因组草图的大小和菌株中基因的数目,并对相关菌株进行了讨论。还针对该菌株中鉴定出的独特基因,提供了对该菌株堆肥潜力的科学见解。使用rDNA区域的系统发育分析已确认该菌株在子囊菌家族中的位置。公开阅读原始资料,并提供全基因组蛋白质组图谱,以促进对该生物的进一步研究。通过杂交方法对古细菌耶尔森菌vka1菌株进行测序,并从头开始重新装配读段。评估了基因组草图的大小和菌株中基因的数目,并对相关菌株进行了讨论。还针对该菌株中鉴定出的独特基因,提供了对该菌株堆肥潜力的科学见解。使用rDNA区域的系统发育分析已确认该菌株在子囊菌家族中的位置。公开阅读原始资料,并提供全基因组蛋白质组图谱,以促进对该生物的进一步研究。通过杂交方法对古细菌耶尔森菌vka1菌株进行测序,并从头开始重新装配读段。评估了基因组草图的大小和菌株中基因的数目,并对相关菌株进行了讨论。还针对该菌株中鉴定出的独特基因,提供了对该菌株堆肥潜力的科学见解。通过杂交方法对古细菌耶尔森菌vka1菌株进行测序,并从头开始重新装配读段。评估了基因组草图的大小和菌株中基因的数目,并对相关菌株进行了讨论。还针对该菌株中鉴定出的独特基因,提供了对该菌株堆肥潜力的科学见解。通过杂交方法对古细菌耶尔森菌vka1菌株进行测序,并从头开始重新装配读段。评估了基因组草图的大小和菌株中基因的数目,并对相关菌株进行了讨论。还针对该菌株中鉴定出的独特基因,提供了对该菌株堆肥潜力的科学见解。
更新日期:2020-09-30
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