Loggerhead (Caretta caretta; Cc) and green sea (Chelonia mydas; Cm) turtles admitted to rehabilitation facilities may require blood transfusions for supportive treatment of disorders resulting in life-threatening anemia, but, considering the unique erythrocyte chemistry of sea turtles, standardized donor red blood cell (RBC) storage protocols have not been established. Prolonged cold storage and the effects of various anticoagulant-preservative solutions have been associated with increased RBC osmotic fragility across a broad range of species. Increased RBC fragility in stored RBC products has been associated with acute transfusion reactions. The osmotic fragility test is used to measure erythrocyte resistance to hemolysis while being exposed to a series of dilutions of a saline solution. We obtained baseline measurements for osmotic fragility in healthy Cc and Cm. Osmotic fragility testing was performed on samples from 10 Cc to 10 Cm. Fifty percent (50%) RBC hemolysis was identified at a mean NaCl concentration of 0.38% in both species. Results of our study will help guide future studies evaluating optimal storage solutions for sea turtle blood products.

蠵龟( Caretta caretta ; Cc) 和绿海 ( Chelonia mydas; Cm) 进入康复设施的海龟可能需要输血来支持治疗导致危及生命的贫血的疾病，但是，考虑到海龟独特的红细胞化学，尚未建立标准化的供体红细胞 (RBC) 储存协议。长期冷藏和各种抗凝血防腐剂溶液的作用与广泛物种的红细胞渗透脆性增加有关。储存的红细胞产品中红细胞脆性增加与急性输血反应有关。渗透脆性试验用于测量红细胞在暴露于一系列稀释的盐水溶液中时对溶血的抵抗力。我们获得了健康 Cc 和 Cm 渗透脆性的基线测量值。对 10 Cc 至 10 Cm 的样品进行渗透脆性测试。在 0.38% 的平均 NaCl 浓度下，在两个物种中都发现了 50% (50%) 的 RBC 溶血。我们的研究结果将有助于指导未来评估海龟血产品最佳储存解决方案的研究。