Equine endometrial and adipose mesenchymal stem cells (eMSCs and aMSCs, respectively) were isolated from the same donors of thoroughbred mares. The cells displayed characteristic features of MSCs, including trilineage mesodermal and also neurogenic differentiation. We evaluated the influence of cellular origin on their transcriptome profile. Cellular RNA was isolated and sequenced and extracellular vesicles (EVs) were obtained from conditioned medium of cells cultured in medium depleted of EVs, and their microRNA (miRNA) cargo analyzed by sequencing. Differential expression of mRNAs and EV-miRNA was analyzed, as well as pathways and processes most represented in each cell origin. mRNA reads from all expressed genes clustered according to the cellular origin. A total of 125 up- and 51 downregulated genes were identified and 31 differentially expressed miRNAs. Based on mRNA sequencing, endometrial MSCs strongly upregulated genes involved in the Hippo, transforming growth factor beta, and pluripotency signaling pathways. Alongside with this, pathways involved in extracellular matrix reorganization were the most represented in the miRNA cargo of EVs secreted by eMSCs. The niche from which MSCs originated defined the transcriptomic signature of the cells, including the secretion of lineage-specific loaded EV to ensure proper communication and homeostasis. Identification and testing their biological functions can provide new tools for the therapeutic use of horse MSC.

中文翻译：

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来自同一供体的马脂肪和子宫内膜间充质干细胞的细胞外囊泡的独特细胞转录组特征和 MicroRNA 货物

马子宫内膜和脂肪间充质干细胞（分别为eMSCs和aMSCs）是从相同的纯种母马供体中分离出来的。这些细胞表现出 MSCs 的特征，包括三系中胚层和神经源性分化。我们评估了细胞来源对其转录组谱的影响。细胞 RNA 被分离和测序，细胞外囊泡 (EV) 是从在耗尽 EV 的培养基中培养的细胞的条件培养基中获得的，并通过测序分析它们的 microRNA (miRNA) 货物。分析了 mRNA 和 EV-miRNA 的差异表达，以及在每个细胞来源中最具代表性的途径和过程。根据细胞来源聚集的所有表达基因的 mRNA 读数。共鉴定出 125 个上调基因和 51 个下调基因，以及 31 个差异表达的 miRNA。基于 mRNA 测序，子宫内膜间充质干细胞强烈上调参与 Hippo、转化生长因子 β 和多能性信号通路的基因。除此之外，参与细胞外基质重组的途径在 eMSCs 分泌的 EVs 的 miRNA 货物中最具代表性。MSCs 起源的生态位定义了细胞的转录组学特征，包括分泌谱系特异性负载的 EV，以确保适当的通信和体内平衡。鉴定和测试它们的生物学功能可以为马 MSC 的治疗用途提供新的工具。和多能性信号通路。除此之外，参与细胞外基质重组的途径在 eMSCs 分泌的 EVs 的 miRNA 货物中最具代表性。MSCs 起源的生态位定义了细胞的转录组学特征，包括分泌谱系特异性负载的 EV，以确保适当的通信和体内平衡。鉴定和测试它们的生物学功能可以为马 MSC 的治疗用途提供新的工具。和多能性信号通路。除此之外，参与细胞外基质重组的途径在 eMSCs 分泌的 EVs 的 miRNA 货物中最具代表性。MSCs 起源的生态位定义了细胞的转录组学特征，包括分泌谱系特异性负载的 EV，以确保适当的通信和体内平衡。鉴定和测试它们的生物学功能可以为马 MSC 的治疗用途提供新的工具。