Comparative Proteomic Profiling of 3T3-L1 Adipocyte Differentiation Using SILAC Quantification,Journal of Proteome Research

当前位置： X-MOL 学术 › J. Proteome Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Comparative Proteomic Profiling of 3T3-L1 Adipocyte Differentiation Using SILAC Quantification
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2020-09-29 , DOI: 10.1021/acs.jproteome.0c00475
Sunkyu Choi ₁ , Neha Goswami ₁ , Frank Schmidt ₁

Affiliation

Adipocyte differentiation is a general physiological process that is also critical for metabolic syndrome. In spite of extensive study in the past two decades, adipogenesis is a still complex cellular process that is accompanied by complicated molecular mechanisms. Here, we performed SILAC-based quantitative global proteomic profiling of 3T3-L1 adipocyte differentiation. We report protein changes to the proteome profiles, with 354 proteins exhibiting significant increase and 56 proteins showing decrease in our statistical analysis. Our results show that adipocyte differentiation is involved not only in metabolic processes by increasing TCA cycle, fatty acid synthesis, lipolysis, acetyl-CoA production, antioxidants, and electron transport, but also in nicotinamide metabolism, cristae formation, mitochondrial protein import, and Ca²⁺ transport into mitochondria and ER. A search for Chromosome-Centric Human Proteome Project (C-HPP) using neXtprot highlighted one protein with a protein existence uncertain (PE5) and 17 proteins as functionally uncharacterized protein existence 1 (uPE1). This study provides quantitative information on proteome changes in adipogenic differentiation, which is helpful in improving our understanding of the processes of adipogenesis.

中文翻译：

使用SILAC定量比较蛋白质组学的3T3-L1脂肪细胞分化

脂肪细胞分化是一般的生理过程，对于代谢综合征也至关重要。尽管在过去的二十年中进行了广泛的研究，但是脂肪形成仍然是一个复杂的细胞过程，并伴随着复杂的分子机制。在这里，我们进行了基于SILAC的3T3-L1脂肪细胞分化的定量全局蛋白质组分析。我们报告了蛋白质组图谱的蛋白质变化，在我们的统计分析中，有354种蛋白质显着增加，有56种蛋白质减少。我们的结果表明，脂肪细胞分化不仅通过增加TCA循环，脂肪酸合成，脂肪分解，乙酰辅酶A的产生，抗氧化剂和电子运输来参与代谢过程，而且还涉及烟酰胺代谢，cr的形成，线粒体蛋白的导入和Ca²⁺转运至线粒体和ER。使用neXtprot进行的以染色体为中心的人类蛋白质组计划（C-HPP）的搜索突出了一种蛋白质，该蛋白质的蛋白质存在不确定性（PE5），而17种蛋白质的功能未知的蛋白质存在1（uPE1）。这项研究提供了有关成脂分化中蛋白质组变化的定量信息，这有助于增进我们对成脂过程的理解。

更新日期：2020-12-04

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11