Infection and insufficient osteointegration are the main causes of orthopedic implant failure. Furthermore, activating favorable inflammation response is vital to the fast osteointegration of implants. Therefore, endowing the implants with multifunctions (antibacterial, anti-inflammation, and pro-osteointegration) is a promising strategy to improve the performance of orthopedic implants. In this study, a Zn-contained polydopamine (PDA) film was fabricated on AZ31 alloy. The film possessed a stable Zn ion release in 14 days. The results of electrochemical analysis implied the favorable corrosion protection of the film, and thus, leading to a suitable hemolysis ratio (below 1%). The in vitro antibacterial assessment revealed that the film exhibited excellent resistance against Staphylococcus aureus (nearly 100%), which can be ascribed to the release of Zn ions. The cell-culture evaluation revealed that the extract of Zn-contained PDA-coated sample can activate RAW264.7 polarization to an anti-inflammatory phenotype, as well as enhance the osteogenic differentiation ability of MC3T3-E1. Additionally, the femoral osteomyelitis model indicated that the as-prepared film had a high antibacterial capability at early stage of the implantation, and showed better osteogenesis and osteointegration after 8 weeks of implantation. With favorable antibacterial, anti-inflammation, and pro-osteogenesis abilities, the novel designed Zn-contained PDA film is promising to be used in Mg-based orthopedic implants.

中文翻译：

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镁合金上含锌的贻贝启发膜，可抑制细菌感染并促进骨骼再生

感染和骨整合不足是造成整形外科植入物失败的主要原因。此外，激活良好的炎症反应对于植入物的快速骨整合至关重要。因此，赋予植入物多种功能（抗菌，抗发炎和促骨整合）是改善骨科植入物性能的一种有前途的策略。在这项研究中，在AZ31合金上制备了含锌的聚多巴胺（PDA）膜。该膜在14天内具有稳定的Zn离子释放。电化学分析的结果暗示了膜的良好的腐蚀保护，因此导致合适的溶血率（低于1％）。的体外抗菌评估表明，该膜表现出优异的抵抗金黄色葡萄球菌（接近100％），这可以归因于Zn离子的释放。细胞培养评估表明，含Zn的PDA涂层样品的提取物可以将RAW264.7极化激活为抗炎表型，并增强MC3T3-E1的成骨分化能力。另外，股骨骨髓炎模型表明，所制备的膜在植入早期具有较高的抗菌能力，并且在植入8周后显示出更好的成骨性和骨整合性。具有良好的抗菌，消炎和促成骨能力，新颖设计的含锌PDA膜有望用于基于Mg的骨科植入物中。