Repair and reconstruction of large bone defect were often difficult, and bone substitute materials, including autogenous bone, allogenic bone and artificial bone, were common treatment strategies. The key to elucidate the clinical effect of these bone repair materials was to study their osteogenic capacity and immunotoxicological compatibility. In this paper, the mechanical properties, micro-CT imaging analysis, digital image analysis and histological slice analysis of the three bone grafts were investigated and compared after different time points of implantation in rat femur defect model. Autogenous bone and biphasic calcium phosphate particular artificial bone containing 61.4% HA and 38.6% β-tricalcium phosphate with 61.64% porosity and 0.8617 ± 0.0068 g/cm³ density (d ≤ 2 mm) had similar and strong bone repair ability, but autogenous bone implant materials caused greater secondary damage to experimental animals; allogenic bone exhibited poor bone defect repair ability. At the early stage of implantation, the immunological indexes such as Immunoglobulin G, Immunoglobulin M concentration and CD4 cells’ population of allogenic bone significantly increased in compared with those of autologous bone and artificial bone. Although the repair process of artificial bone was relatively inefficient than autologous bone graft, the low immunotoxicological indexes and acceptable therapeutic effects endowed it as an excellent alternative material to solve the problems with insufficient source and secondary trauma of autogenous bone.

中文翻译：

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自体，同种异体骨和人工骨替代物对大鼠股骨缺损模型骨再生和免疫毒性的比较研究

大骨缺损的修复和重建通常很困难，包括自体骨，同种异体骨和人造骨在内的骨替代材料是常见的治疗策略。阐明这些骨修复材料的临床效果的关键是研究其成骨能力和免疫毒性。本文研究了三种股骨缺损模型在不同植入时间点后的力学性能，显微CT成像分析，数字图像分析和组织切片分析，并进行了比较。自体骨和双相磷酸钙特别是人造骨，含有61.4％的HA和38.6％的β-磷酸三钙，孔隙度为61.64％，密度为0.8617±0.0068 g / cm ³（d ≤2 mm）具有相似且强大的骨修复能力，但自体骨植入材料对实验动物造成更大的继发性损伤；同种异体骨显示出较差的骨缺损修复能力。与自体骨和人工骨相比，在植入初期，免疫指标如免疫球蛋白G，免疫球蛋白M浓度和同种异体骨的CD4细胞数量显着增加。尽管人造骨的修复过程比自体骨移植的效率相对较低，但是低的免疫毒理学指标和可接受的治疗效果使其成为解决自体骨来源和继发性创伤不足的极好的替代材料。