Metallic nanoparticles (NPs) have been accepted for various applications ranging from cosmetics to medicine. However, no method has been established in the scientific community that is capable of analyzing various metals, sizes, and levels of exposures without the concern of background chemical contaminations. We present here a system utilizing soft-landing ion mobility (SLIM) exposures of laser ablated metallic clusters capable of operating pressures of reduced vacuum (1 Torr) up to ambient (760 Torr) in the presence of a buffer gas. Clusters experience kinetic energies of less than 1 eV upon exiting the SLIM, allowing for the exposure of NPs to take place in a passive manner. While there is no mass-selection of cluster sizes in this work, it does show for the first time the creation and soft-landing of nanoclusters at ambient pressures. Factors such as area coverage and percentage distribution were studied, as well as the different effects that varying surfaces may cause in the agglomeration of the clusters. Furthermore, the system was successfully used to study the effects of silver nanoparticle exposure and determine the specific organs the NPs accumulate in using zebrafish (Danio rerio) as a model organism. This method provides a novel way to synthesize NPs and expose biological organisms for various toxicological analysis.

中文翻译：

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用于使用斑马鱼的毒理学研究应用的金属纳米颗粒生产和暴露/沉积系统

金属纳米粒子 (NPs) 已被接受用于从化妆品到医学的各种应用。然而，科学界还没有建立能够分析各种金属、尺寸和暴露水平而无需担心背景化学污染的方法。我们在此介绍了一种系统，该系统利用激光烧蚀金属簇的软着陆离子迁移率 (SLIM) 曝光，该系统能够在缓冲气体存在的情况下将真空 (1 Torr) 降低到环境 (760 Torr) 的压力。离开 SLIM 后，团簇的动能小于 1 eV，从而允许 NP 以被动方式暴露。虽然在这项工作中没有对簇大小进行质量选择，但它确实首次展示了在环境压力下纳米簇的产生和软着陆。研究了面积覆盖率和百分比分布等因素，以及不同表面可能对集群聚集产生的不同影响。此外，该系统成功地用于研究银纳米粒子暴露的影响，并确定纳米粒子在使用斑马鱼 (Danio rerio) 作为模式生物时积累的特定器官。该方法提供了一种合成纳米颗粒和暴露生物有机体以进行各种毒理学分析的新方法。该系统成功地用于研究银纳米颗粒暴露的影响，并确定纳米颗粒在使用斑马鱼 (Danio rerio) 作为模式生物时积累的特定器官。该方法提供了一种合成纳米颗粒和暴露生物有机体以进行各种毒理学分析的新方法。该系统成功地用于研究银纳米颗粒暴露的影响，并确定纳米颗粒在使用斑马鱼 (Danio rerio) 作为模式生物时积累的特定器官。该方法提供了一种合成纳米颗粒和暴露生物有机体以进行各种毒理学分析的新方法。