In the current investigation, titanium-dioxide nanoparticles (TiO₂-NPs) are prepared through a sol–gel method at varying pH values of 6, 8, and 10. The effects of pH on the preparation of TiO₂-NPs and their structural, morphological, and compositional variations were extensively studied by P-XRD, TEM, FE-SEM, and EDS techniques. The calculated optical bandgap (E_g) was in the range of 3.40 eV to 3.15 eV, with an increase in pH, and was observed by the K–M method. The photocatalytic activity of TiO₂-NPs is evident from that the degradation of the reactive black 5 (RB5) dye shows 96% photocatalytic activity at 45 min under UV irradiation. The photocatalytic activity of TiO₂-NPs is pH-sensitive. The photocatalytic activity of TiO₂-NPs increases if the reaction is performed under acidic conditions. Moreover, all the synthesized TiO₂-NPs obey pseudo-first-order kinetics. TiO₂-NPs could be coated on medical implants to prevent the growth of micro-organisms and facilitate vascular protection. In this context, 100 µg/ml, 200 µg/ml, and 300 μg/ml of TiO₂-NPs were coated on materials as a treatment to study the incorporation of bacteria by flow cytometry analysis. Among the treatments, a statistical increase in E. coli and B. subtilis cell incorporation by around 17.3% and 24% was observed in the groups treated with 200 μg/ml of TiO₂-NPs synthesized at pH 6. Furthermore, the cytotoxicity of TiO₂-NPs on the osteoblasts cell line, C3H10T1/2, was analyzed for cell viability and proliferation by MTT and BrdU assays, respectively. TiO₂-NPs were synthesized at different pH values and a high percentage viability is seen in the samples synthesized at a pH of 6.

中文翻译：

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pH依赖的TiO2纳米颗粒的合成对细菌生长抑制和成骨细胞增殖有影响

在当前的研究中，通过溶胶-凝胶法在6、8和10的不同pH值下制备了二氧化钛纳米颗粒（TiO ₂ -NPs）。pH对TiO ₂ -NPs的制备及其结构的影响，形态和组成变化已通过P-XRD，TEM，FE-SEM和EDS技术进行了广泛研究。计算的光学带隙（E _g）在3.40 eV至3.15 eV的范围内，随着pH值的增加，可以通过K–M方法观察到。TiO ₂ -NPs的光催化活性从反应性黑5（RB5）染料的降解显示了在UV辐射下45分钟时96％的光催化活性。TiO ₂的光催化活性-NPs对pH敏感。如果反应在酸性条件下进行，则TiO ₂ -NPs的光催化活性增加。此外，所有合成的TiO ₂ -NPs均服从拟一级动力学。可以将TiO ₂ -NPs涂覆在医疗植入物上，以防止微生物的生长并促进血管保护。在这种情况下，将TiO ₂ -NPs 100 µg / ml，200 µg / ml和300 µg / ml涂覆在材料上，作为通过流式细胞术分析研究细菌掺入的处理方法。在这些治疗中，大肠杆菌和枯草芽孢杆菌的统计增加在用200处理的组中观察到了约17.3％和24％的细胞掺入μ微克/毫升的TiO _2个在pH 6。此外合成-nps，TiO 2的细胞毒性_2个-nps对成骨细胞的细胞系，C3H10T1 / 2，通过MTT和BrdU分析分别分析细胞活力和增殖。TiO ₂ -NPs是在不同的pH值下合成的，在pH为6的合成样品中可以看到较高的生存率。