This study was conducted to examine the effects of the replacement of fish meal with defatted and fermented soybean meals on the growth performance, apparent nutrient digestibility, bile acid levels, and digestive enzyme activity of pompano Trachinotus blochii (Lacepède, 1801). Four diets were formulated to replace 40% of fish meal with defatted soybean meal (SBM), SBM fermented by Bacillus subtilis TH2 (FSBM1) or SBM fermented by B. subtilis B3 (FSBM2). The diets are denoted as follows: FMD (fish meal-based diet, used as a Control), SBMD (fish meal replaced by SBM diet), FSBM1D (fish meal replaced by FSBM1 diet), and FSBM2D (fish meal replaced by FSBM2 diet). Thirty fingerling pompanos with an initial body weight of 15.3 ± 0.3 g were allocated to each of eight indoor polyvinyl chloride tanks (500 L capacity), with two replicate tanks per dietary treatment. For 8 weeks, fish were hand-fed the experimental diets to apparent satiation twice daily. The final body weight, weight gain, specific growth rate, and feed conversion ratio of fish fed SBMD and FSBM1D were significantly inferior to those in fish fed FMD (P<0.05). These parameters were not significantly different between the FSBM2D and FMD experimental groups (P>0.05). Fish fed SBMD showed significantly lower plasma total cholesterol, whole body lipids, intestinal total bile acids and lipase activity than those fed FMD, whereas no significant differences were observed among fish fed FSBM1D, FSBM2D, and FMD. Trypsin activity and protein apparent digestibility coefficient were not significantly affected by the experimental diets. However, lipid apparent digestibility coefficient was significantly lower in fish fed SBMD and FSBM1D rather than FMD. There was no significant difference in lipid apparent digestibility coefficient between the FMD and FSBM2D experimental groups. The results indicated that SBM decreased bile acid levels, lipase activity, lipid digestibility, and growth performance in pompano fish. These parameters were increased by the inclusion of B. subtilis B3 fermented SBM in the diet, thus suggesting that fermentation of SBM with B. subtilis B3 may be an effective way to improve bile acid levels, lipase activity, lipid digestibility, and growth performance of pompano fed an SBM-based diet.

中文翻译：

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po鱼Trachinotus blochii（Lacepède，1801）日粮中的脱脂发酵豆粕代替鱼粉

进行该研究以检查替代鱼粉的对生长性能脱脂和发酵大豆膳食，表观营养物质消化率，胆汁酸水平，和鲳的消化酶活性的影响鲳blochii（Lacepède，1801）。配制了四种日粮，用脱脂豆粕（SBM），枯草芽孢杆菌TH2发酵的SBM（FSBM1）或枯草芽孢杆菌发酵的SBM代替40％的鱼粉。B3（FSBM2）。饮食如下：FMD（基于鱼粉的饮食，用作对照），SBMD（被SBM饮食替代的鱼粉），FSBM1D（由FSBM1饮食替代的鱼粉）和FSBM2D（由FSBM2饮食替代的鱼粉） ）。将30个初始体重为15.3±0.3 g的鱼种po鱼分配到八个室内聚氯乙烯罐（容量为500 L）中的每个罐中，每个饮食处理重复两个罐。连续8周，每天两次给鱼喂饲实验性饮食，使其明显饱食。饲喂SBMD和FSBM1D的鱼的最终体重，体重增加，比生长率和饲料转化率显着低于饲喂F​​MD的鱼（P <0.05）。FSBM2D和FMD实验组之间这些参数没有显着差异（P> 0.05）。饲喂SBMD的鱼显示血浆总胆固醇，全身脂质，肠道总胆汁酸和脂肪酶活性明显低于饲喂F​​MD的鱼，而饲喂FSBM1D，FSBM2D和FMD的鱼之间没有观察到显着差异。实验饮食对胰蛋白酶活性和蛋白质表观消化率没有显着影响。但是，饲喂SBMD和FSBM1D的鱼的脂质表观消化率系数明显低于FMD。FMD和FSBM2D实验组之间的脂质表观消化系数没有显着差异。结果表明，SBM降低了mp鱼的胆汁酸水平，脂肪酶活性，脂质消化率和生长性能。通过增加以下参数来增加这些参数 饲喂FSBM1D，FSBM2D和FMD的鱼的肠道总胆汁酸和脂肪酶活性均高于饲喂FMD的鱼。实验饮食对胰蛋白酶活性和蛋白质表观消化率没有显着影响。但是，饲喂SBMD和FSBM1D的鱼的脂质表观消化率系数明显低于FMD。FMD和FSBM2D实验组之间的脂质表观消化系数没有显着差异。结果表明，SBM降低了mp鱼的胆汁酸水平，脂肪酶活性，脂质消化率和生长性能。通过增加以下参数来增加这些参数 饲喂FSBM1D，FSBM2D和FMD的鱼的肠道总胆汁酸和脂肪酶活性均高于饲喂FMD的鱼。实验饮食对胰蛋白酶活性和蛋白质表观消化率没有显着影响。但是，饲喂SBMD和FSBM1D的鱼的脂质表观消化率系数明显低于FMD。FMD和FSBM2D实验组之间的脂质表观消化系数没有显着差异。结果表明，SBM降低了mp鱼的胆汁酸水平，脂肪酶活性，脂质消化率和生长性能。通过增加以下参数来增加这些参数 实验饮食对胰蛋白酶活性和蛋白质表观消化率没有显着影响。但是，饲喂SBMD和FSBM1D的鱼的脂质表观消化率系数明显低于FMD。FMD和FSBM2D实验组之间的脂质表观消化系数没有显着差异。结果表明，SBM降低了mp鱼的胆汁酸水平，脂肪酶活性，脂质消化率和生长性能。通过增加以下参数来增加这些参数 实验饮食对胰蛋白酶活性和蛋白质表观消化率没有显着影响。但是，饲喂SBMD和FSBM1D的鱼的脂质表观消化率系数明显低于FMD。FMD和FSBM2D实验组之间的脂质表观消化系数没有显着差异。结果表明，SBM降低了mp鱼的胆汁酸水平，脂肪酶活性，脂质消化率和生长性能。通过增加以下参数来增加这些参数 FMD和FSBM2D实验组之间的脂质表观消化系数没有显着差异。结果表明，SBM降低了mp鱼的胆汁酸水平，脂肪酶活性，脂质消化率和生长性能。通过增加以下参数来增加这些参数 FMD和FSBM2D实验组之间的脂质表观消化系数没有显着差异。结果表明，SBM降低了mp鱼的胆汁酸水平，脂肪酶活性，脂质消化率和生长性能。通过增加以下参数来增加这些参数枯草芽孢杆菌B3使日粮中的SBM发酵，因此表明用枯草芽孢杆菌B3发酵SBM可能是提高以SBM饲料喂养的po鱼的胆汁酸水平，脂肪酶活性，脂质消化率和生长性能的有效方法。