The major objectives of this study were to investigate the effects of silver nanoparticles– gelatin (AgNPs) on the physical and chemical properties of gelatin/alginate (Gel/Alg) scaffolds and the bone-promoting effect of AgNP–Gel/Alg scaffolds. Gel/Alg scaffolds consisting of 0 μM, 200 μM, 400 μM, and 600 μM AgNPs were prepared. SEM was used to evaluate the physical properties of the scaffolds. A CCK-8 assay was performed to determine the cell proliferation activity, and Micro-CT and histological analysis were used to assess the osteogenic effect. The pore size, porosity, and the water absorption and degradation rates of AgNP–Gel/Alg scaffolds were found to be increased compared with those of Gel/Alg scaffolds (control group). CCK-8 showed that cell proliferation activity in the 200 μM group was significantly higher than that in the control group. Micro-CT analysis showed that there was more new bone around AgNP–Gel/Alg than the control group, and the amount of bone formation in the 200 μM group was significantly higher than that in the other groups. Masson staining showed that numerous collagen fibers had proliferated around the AgNP–Gel/Alg scaffold and tended to thicken over time. AgNP–Gel/Alg scaffolds promoted the repair of skull defects in New Zealand rabbits and exerted a marked osteogenic effect in vivo. The 200 μM AgNP–Gel/Alg scaffold was shown to be more suitable for bone tissue engineering materials.

中文翻译：

---

使用纳米银-明胶/藻酸盐支架修复颅骨缺损

这项研究的主要目的是研究银纳米颗粒-明胶（AgNPs）对明胶/藻酸盐（Gel / Alg）支架的物理和化学性质的影响以及AgNP–Gel / Alg支架对骨的促进作用。制备了由0μM，200μM，400μM和600μMAgNP组成的Gel / Alg支架。SEM用于评估支架的物理性质。进行CCK-8测定以确定细胞增殖活性，并且使用Micro-CT和组织学分析来评估成骨作用。发现AgNP–Gel / Alg支架的孔径，孔隙率以及吸水率和降解率比凝胶/ Alg支架（对照组）增加。CCK-8表明200μM组的细胞增殖活性明显高于对照组。Micro-CT分析显示，AgNP–Gel / Alg周围的新骨比对照组多，并且200μM组的骨形成量显着高于其他组。Masson染色显示，大量的胶原纤维在AgNP–Gel / Alg支架周围增生，并随着时间的推移趋于增厚。AgNP–Gel / Alg支架促进了新西兰兔颅骨缺损的修复，并在体内发挥了显着的成骨作用。已证明200μMAgNP–Gel / Alg支架更适合于骨组织工程材料。Masson染色显示，大量的胶原纤维在AgNP–Gel / Alg支架周围增生，并随着时间的推移趋于增厚。AgNP–Gel / Alg支架促进了新西兰兔颅骨缺损的修复，并在体内发挥了显着的成骨作用。已证明200μMAgNP–Gel / Alg支架更适合于骨组织工程材料。Masson染色显示，大量的胶原纤维在AgNP–Gel / Alg支架周围增生，并随着时间的推移趋于增厚。AgNP–Gel / Alg支架促进了新西兰兔颅骨缺损的修复，并在体内发挥了显着的成骨作用。已证明200μMAgNP–Gel / Alg支架更适合于骨组织工程材料。