In this study, we examined the association between antimicrobial resistance, CRISPR/Cas systems and virulence with phage susceptibility in Acinetobacter baumannii and investigated draft genomes of phage susceptible multidrug resistant A. baumannii strains from Thailand. We investigated 230 A. baumannii strains using 17 lytic A. baumannii phages and the phage susceptibility was 46.5% (107/230). Phage susceptibility was also associated with resistance to numerous antibiotics (p-value < 0.05). We also found association between biofilm formation and the presence of ompA gene among phage susceptible A. baumannii strains (p-value < 0.05). A. baumannii isolates carrying cas5 or combinations of two or three other cas genes, showed a significant increase in phage resistance. Whole-genome sequences of seven phage susceptible A. baumannii isolates revealed that six groups of antibiotic resistance genes were carried by all seven phage susceptible A. baumannii. All strains carried biofilm associated genes and two strains harbored complete prophages, acquired copper tolerance genes, and CRISPR-associated (cas) genes. In conclusion, our data exhibits an association between virulence determinants and biofilm formation among phage susceptible A. baumannii strains. These data help to understand the bacterial co-evolution with phages.

在这项研究中，我们研究了鲍曼不动杆菌中抗菌素耐药性、CRISPR/Cas 系统和毒力与噬菌体敏感性之间的关联，并研究了来自泰国的噬菌体易感多重耐药鲍曼不动杆菌菌株的基因组草图。我们使用 17 种溶解性鲍曼不动杆菌噬菌体研究了 230株鲍曼不动杆菌菌株，噬菌体敏感性为 46.5% (107/230)。噬菌体敏感性还与对多种抗生素的抗性有关（p值 < 0.05）。我们还发现噬菌体敏感的鲍曼不动杆菌菌株中生物膜形成与ompA基因的存在之间存在关联（p-值 < 0.05）。携带cas5或两个或三个其他cas基因组合的鲍曼不动杆菌分离株显示出噬菌体抗性的显着增加。七个噬菌体敏感鲍曼不动杆菌的全基因组序列显示，所有七个噬菌体敏感鲍曼不动杆菌都携带六组抗生素抗性基因。所有菌株都携带生物膜相关基因，两个菌株携带完整的原噬菌体、获得性铜耐受基因和 CRISPR 相关 ( cas ) 基因。总之，我们的数据表明，噬菌体易感鲍曼不动杆菌的毒力决定因素与生物膜形成之间存在关联。菌株。这些数据有助于了解细菌与噬菌体的共同进化。