Abstract Biofilm fluoride reservoirs may be a source of fluoride to the fluid phase during a sugar challenge reducing tooth mineral loss. However, the evidence for that is conflicting and has not been studied in biofilms containing different fluoride levels. In order to test fluoride release from biofilms with distinct fluoride concentrations, biofilms were grown in situ exposed to a combination of placebo, calcium and fluoride rinses forming biofilms with no (fluoride-free rinses), low (fluoride-only rinses) or high (calcium followed by fluoride rinses) fluoride concentrations, and collected before and 5 min after a sucrose challenge. Rinsing with fluoride increased fluoride concentration in the biofilm (p < 0.05), mainly when a calcium pre-rinse was used before the fluoride (p < 0.05). However, after a sugar challenge, no significant increase in the biofilm fluid fluoride concentration was observed, even in the fluoride-rich biofilms (p > 0.05). Fluoride-rich biofilms do not release fluoride to the fluid phase during a sugar challenge.

中文翻译：

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关于生龋挑战期间生物膜储层中氟化物的释放：一项原位研究

摘要 生物膜氟化物储层可能是糖挑战期间流体相氟化物的来源，减少牙齿矿物质流失。然而，这方面的证据是相互矛盾的，并且尚未在含有不同氟化物水平的生物膜中进行研究。为了测试具有不同氟化物浓度的生物膜的氟化物释放，生物膜在暴露于安慰剂、钙和氟化物冲洗液的组合中原位生长，形成无（无氟冲洗液）、低（仅含氟冲洗液）或高（含氟冲洗液）的生物膜（钙，然后用氟化物冲洗）氟化物浓度，并在蔗糖挑战之前和之后 5 分钟收集。用氟化物冲洗会增加生物膜中的氟化物浓度 (p < 0.05)，主要是在氟化物之前使用钙预冲洗时 (p < 0.05)。然而，经过糖的挑战，没有观察到生物膜流体氟化物浓度的显着增加，即使在富含氟化物的生物膜中也是如此（p > 0.05）。在糖挑战期间，富含氟化物的生物膜不会将氟化物释放到流体相中。