Abstract

A novel sandwich-type electrochemiluminescence (ECL) immunosensor was successfully fabricated for the ultrasensitive detection of avian leukosis virus subgroup J (ALV-J). The immunosensor used a glassy carbon electrode decorated with gold nanoparticles (Au NPs), primary antibodies (Ab₁) and bovine serum albumin as the sensor platform. For the signal label, three-dimensional flower-like MnO₂ clusters modified with polyethyleneimine and palladium nanoparticles (MnO₂–PEI–Pd nanocomposite) were used as the novel carrier of Ru(bpy)₃Cl₂ and secondary antibodies (Ab₂). Under optimal conditions, the ECL immunosensor offered a linear relationship between the ECL signal and the logarithm of the ALV-J concentration in the range from 10^1.98 to 10^4.30 TCID₅₀/mL with a detection limit of 10^1.90 TCID₅₀/mL (based upon a signal-to-noise ratio of 3). In addition, the immunosensor showed excellent stability, selectivity and reproducibility, thus offering an effective tool for the quantitative detection of ALV-J in clinical applications.

摘要

新型三明治型电化学发光（ECL）免疫传感器已成功地用于禽白血病J亚组（ALV-J）的超灵敏检测。免疫传感器使用装饰有金纳米颗粒（Au NPs），一抗（Ab ₁）和牛血清白蛋白的玻璃碳电极作为传感器平台。对于信号标签，使用聚乙烯亚胺和钯纳米粒子（MnO ₂ -PEI-Pd纳米复合材料）修饰的三维花状MnO ₂簇用作Ru（bpy）₃ Cl ₂和二抗（Ab ₂）。在最佳条件下，ECL免疫传感器在ECL信号和ALV-J浓度的对数之间提供线性关系，范围为10 ^1.98到10 ^4.30 TCID ₅₀ / mL，检测极限为10 ^1.90 TCID ₅₀ / mL（基于信噪比为3时）。另外，该免疫传感器显示出优异的稳定性，选择性和可重复性，从而为临床应用中的ALV-J定量检测提供了有效的工具。