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Neuroprotective role of chrysin‐loaded poly(lactic‐co‐glycolic acid) nanoparticle against kindling‐induced epilepsy through Nrf2/ARE/HO‐1 pathway
Journal of Biochemical and Molecular Toxicology ( IF 3.2 ) Pub Date : 2020-09-29 , DOI: 10.1002/jbt.22634 Ying Zhang 1 , Jing Zhao 2, 3 , Obaid Afzal 4 , Imran Kazmi 5 , Fahad A Al-Abbasi 5 , Abdulmalik S A Altamimi 4 , Zhen Yang 6
Journal of Biochemical and Molecular Toxicology ( IF 3.2 ) Pub Date : 2020-09-29 , DOI: 10.1002/jbt.22634 Ying Zhang 1 , Jing Zhao 2, 3 , Obaid Afzal 4 , Imran Kazmi 5 , Fahad A Al-Abbasi 5 , Abdulmalik S A Altamimi 4 , Zhen Yang 6
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Chrysin is the major bioactive compound of blue passionflower, an important medicinal plant used in traditional herbal formulations since ancient times. In the present study, we report that chrysin nanoparticles (chrysin NPs) protect Wistar rats against kindling‐induced epilepsy. Nanoparticles of sizes less than 150 nm with a spherical shape were prepared using poly(d,l‐lactic‐co‐glycolic acid) and polyvinyl alcohol, respectively, as polymer and stabilizer. Rats were injected with subconvulsive doses of pentylenetetrazole (PTZ) (35 mg/kg, intraperitoneal) every second day, with 22 injections in total, and on the same days, they received protective doses of the chrysin NPs (5 and 10 µg/mL, PO), respectively, 45 min before each PTZ injection. After the last PTZ injection, an average of thirteen seizure scores was recorded. Animals were killed by decapitation 24 h after a seizure. The cortex and hippocampus were removed and stored in liquid nitrogen for determining oxidative stress terminal deoxynucleotidyl transferase dUTP nick‐end labeling assay, histopathology, and reverse transcription‐polymerase chain reaction for messenger RNA expression. The result showed chrysin NPs treatment has counteracted oxidative stress, reduced neuronal apoptosis, and upregulated nuclear factor erythroid 2‐related factor 2 (Nrf2), heme oxygenase‐1 (HO‐1), and NAD(P)H quinone oxidoreductase 1. In conclusion, our findings demonstrate that the neuroprotective effect of chrysin NPs against kindling‐induced epilepsy might be escorted by the alleviation of oxidative stress through the Nrf2/antioxidant response element/HO‐1 pathway signal pathway.
中文翻译：
菊花蛋白负载的聚乳酸-乙醇酸纳米颗粒通过Nrf2 / ARE / HO-1途径对点燃诱发的癫痫的神经保护作用
菊花是蓝色西番莲的主要生物活性化合物，是自古以来用于传统草药配方的重要药用植物。在本研究中，我们报道了链纳米粒（chrysin NPs）保护Wistar大鼠免于点燃引发的癫痫。使用poly（d，l）制备尺寸小于150 nm的球形球形纳米颗粒（乳酸-乙醇酸）和聚乙烯醇分别作为聚合物和稳定剂。每隔第二天向大鼠注射一次惊厥剂量的戊四氮（PTZ）（35 mg / kg，腹膜内），共注射22次，并在同一天接受保护剂量的Chrysin NP（5和10 µg / mL） ，PO），分别在每次PTZ注射前45分钟。最后一次PTZ注射后，记录平均13次癫痫发作分数。癫痫发作24小时后，通过斩首处死动物。取出皮层和海马并储存在液氮中，以测定氧化应激末端脱氧核苷酸转移酶dUTP缺口末端标记测定，组织病理学和逆转录聚合酶链反应以检测信使RNA的表达。
更新日期：2020-09-29
中文翻译：
菊花蛋白负载的聚乳酸-乙醇酸纳米颗粒通过Nrf2 / ARE / HO-1途径对点燃诱发的癫痫的神经保护作用
菊花是蓝色西番莲的主要生物活性化合物，是自古以来用于传统草药配方的重要药用植物。在本研究中，我们报道了链纳米粒（chrysin NPs）保护Wistar大鼠免于点燃引发的癫痫。使用poly（d，l）制备尺寸小于150 nm的球形球形纳米颗粒（乳酸-乙醇酸）和聚乙烯醇分别作为聚合物和稳定剂。每隔第二天向大鼠注射一次惊厥剂量的戊四氮（PTZ）（35 mg / kg，腹膜内），共注射22次，并在同一天接受保护剂量的Chrysin NP（5和10 µg / mL） ，PO），分别在每次PTZ注射前45分钟。最后一次PTZ注射后，记录平均13次癫痫发作分数。癫痫发作24小时后，通过斩首处死动物。取出皮层和海马并储存在液氮中，以测定氧化应激末端脱氧核苷酸转移酶dUTP缺口末端标记测定，组织病理学和逆转录聚合酶链反应以检测信使RNA的表达。
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