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A novel, rapid technique for clearing leaf tissues
Applications in Plant Sciences ( IF 2.7 ) Pub Date : 2020-09-30 , DOI: 10.1002/aps3.11391
Emmanuel García-Gutiérrez 1 , Fernando Ortega-Escalona 2 , Guillermo Angeles 2
Affiliation  

Clearing leaves is a highly useful practice for many taxonomic, ecological, physiological, and eco‐physiological aspects of research. Using traditional methods, the procedure for clearing a leaf (referred to as diaphanization) can take several days or even weeks. In our laboratory we developed a technique, originally used for dissociating wood, that yields excellent epidermal and leaf venation preparations clearly showing the details of epidermal cells, hydathodes, trichomes, leaf margins, and leaf venation, in a maximum of three days.

中文翻译:


一种新颖、快速的叶组织清理技术



清理叶子对于许多分类学、生态学、生理学和生态生理学方面的研究来说是一种非常有用的做法。使用传统方法,清理叶子的过程(称为透明化)可能需要几天甚至几周的时间。在我们的实验室中,我们开发了一种最初用于分离木材的技术,可在最多三天内产生出色的表皮和叶脉制备物,清楚地显示表皮细胞、水泡、毛状体、叶缘和叶脉的细节。
更新日期:2020-09-30
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