The stint of the bacterial species is convoluting, but the new algorithms to calculate genome‐to‐genome distance (GGD) and DNA–DNA hybridization (DDH) for comparative genome analysis have rejuvenated the exploration of species and sub‐species characterization. The present study reports the first whole genome sequence of Exiguobacterium profundum PHM11. PHM11 genome consist of ~ 2.92 Mb comprising 48 contigs, 47.93% G + C content. Functional annotations revealed a total of 3033 protein coding genes and 33 non‐protein coding genes. Out of these, only 2316 could be characterized and others reported as hypothetical proteins. The comparative analysis of predicted proteome of PHM11 with five other Exiguobacterium sp. identified 3806 clusters, out of which the PHM11 shared a total of 2723 clusters having 1664 common clusters, 131 singletons and 928 distributed between five species. The pan‐genome analysis of 70 different genomic sequences of Exigubacterium strains devoid of a species taxon was done on the basis of GGD and the DDH which identified eight genomes analogous to the PHM11 at species level and may be characterized as E. profundum. The ANI value and phylogenetic tree analysis also support the same. The results regarding pan‐genome analysis provide a convincing insight for delineation of these eight strains to species.

中文翻译：

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Exiguobacterium的全基因组分析揭示了与Prodigum profundum PHM 11的物种描述和基因组相似性

细菌物种的状况令人费解，但是用于比较基因组分析的计算基因组与基因组距离（GGD）和DNA-DNA杂交（DDH）的新算法使物种和亚种表征的探索重新焕发活力。本研究报告了深部Exiguobacterium PHM11的第一个完整基因组序列。PHM11基因组由〜2.92 Mb组成，包含48个重叠群，G + C含量为47.93％。功能注释揭示了总共3033个蛋白质编码基因和33个非蛋白质编码基因。在这些蛋白质中，只有2316可以鉴定，其他蛋白质则报道为假定蛋白质。PHM11的预测蛋白质组的比较分析与其他五个微小杆菌属菌。确定了3806个集群，其中PHM11共共享2723个集群，其中共有1664个公共集群，131个单例和928个在五个物种之间分布。的70个不同的基因组序列的泛基因组分析Exigubacterium菌株缺乏分类单元被GGD的基础，并且确定了八个基因组在物种水平类似于PHM11，并且可以表征为DDH上完成一个物种的E. profundum。ANI值和系统树分析也支持这一点。有关全基因组分析的结果提供了令人信服的见解，可以将这8个菌株描述为物种。