Correlations between available primary amines, endospore coat thickness, and alkaline glutaraldehyde sensitivity for spores of select Bacillus species,MicrobiologyOpen

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Correlations between available primary amines, endospore coat thickness, and alkaline glutaraldehyde sensitivity for spores of select Bacillus species
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-09-29 , DOI: 10.1002/mbo3.1117
Jacob Kent Player ₁ , Justen Thalmus Despain ₁ , Richard A Robison ₁

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Alkaline glutaraldehyde (GTA) is a high‐level chemical disinfectant/sterilant and has a broad microbial kill spectrum. The precise antimicrobial mechanism of GTA remains debated. GTA kill times are extremely variable across different organisms, illustrating the need for a better understanding of GTA kill mechanisms related to different organisms. A commonly proposed GTA kill mechanism suggests that it works by cross‐linking accessible primary amines on important surface proteins. If true, the antimicrobial activity of GTA may directly correlate to the number of these available functional groups. Bacillus species form highly resistant bacterial endospores that are commonly used as one of the most stringent test organisms for disinfection and sterilization. In this study, we compared the log reduction times of alkaline GTA on spores from 4 Bacillus species to fluorescent profiles generated using Alexa Fluor™ amine‐reactive dyes. GTA kill times were also compared to mean spore coat thicknesses as measured with scanning electron microscopy (SEM). Fluorescence values generated from bound amine‐reactive dye showed a strong, positive correlation to GTA susceptibility, as measured by GTA 6‐log₁₀ reduction times. Spore coat thickness also showed a strong, positive correlation to reduction time values. Results support the hypothesis that GTA kill times are directly related to the number of available primary amines on bacterial endospores. Results also indicated that the killing efficacy of GTA may be influenced by its ability to penetrate the spore coat to reach additional targets, suggesting that damaging important biomolecules beyond surface proteins may be involved in GTA killing mechanisms.

中文翻译：

可用伯胺、内生孢子外壳厚度和选定芽孢杆菌属物种孢子的碱性戊二醛敏感性之间的相关性

碱性戊二醛 (GTA) 是一种高级化学消毒剂/杀菌剂，具有广泛的微生物杀灭谱。GTA 的确切抗菌机制仍有争议。不同生物体的 GTA 杀伤时间差异极大，这说明需要更好地了解与不同生物体相关的 GTA 杀伤机制。一种普遍提出的 GTA 杀伤机制表明，它通过交联重要表面蛋白质上的可及伯胺来发挥作用。如果属实，则 GTA 的抗菌活性可能与这些可用官能团的数量直接相关。芽孢杆菌物种形成高度抗性的细菌内生孢子，通常用作最严格的消毒和灭菌测试生物之一。在本研究中，我们将碱性 GTA 对 4种芽孢杆菌孢子的对数减少时间与使用 Alexa Fluor™ 胺反应性染料产生的荧光谱进行了比较。还将 GTA 杀灭时间与用扫描电子显微镜 (SEM) 测量的平均孢子涂层厚度进行比较。根据 GTA 6-log ₁₀测量，结合胺反应性染料产生的荧光值与 GTA 敏感性呈强正相关减少次数。孢子外壳厚度也与还原时间值呈强正相关。结果支持 GTA 杀灭时间与细菌内生孢子上可用伯胺数量直接相关的假设。结果还表明，GTA 的杀伤效力可能受其穿透孢子外壳以到达其他目标的能力的影响，这表明破坏表面蛋白以外的重要生物分子可能参与 GTA 杀伤机制。

更新日期：2020-11-12

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