The importance of considering the bioavailability of metals in understanding and assessing their toxicity in freshwaters has been recognized for many years. Currently, biotic ligand models (BLMs) are being applied for the derivation and implementation of environmental quality standards (EQS) for metals under the Water Framework Directive in Europe. bio‐met is a simplified tool that was developed for implementing bioavailability‐based EQS for metals in European freshwaters. We demonstrate the reliability of the relationship between the full BLM predictions and the thresholds (hazardous concentration affecting 5% of species [HC5] values) predicted by bio‐met in 3 stages, for the metals copper, nickel, and zinc. First, ecotoxicity data for specific species from laboratory tests in natural waters are compared with predictions by the individual species BLMs included in the full BLMs. Second, the site‐specific HC5 values predicted by bio‐met for the natural waters used for ecotoxicity testing are compared with those provided by the full BLMs. The reliability of both relationships is demonstrated for all 3 metals, with more than 80% of individual species BLM predictions being within a factor of 3 of the experimental results, and 99% of bio‐met local HC5 predictions being within a factor of 2 of the full BLM result. Third, using a larger set of European natural waters in addition demonstrates the reliability of bio‐met over a broad range of water chemistry conditions. bio‐met is therefore an appropriate tool for performing compliance assessments against EQS values in Europe, due to the demonstrated consistency with the toxicity test data. Environ Toxicol Chem 2020;39:2361–2377. © 2020 SETAC

中文翻译：

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证明bio-met确定欧洲金属环境质量标准符合性的可靠性

多年来，人们已经认识到考虑金属的生物利用度对理解和评估其在淡水中的毒性的重要性。目前，根据欧洲《水框架指令》，生物配体模型（BLM）正在用于推导和实施金属的环境质量标准（EQS）。biomet是一种简化的工具，用于在欧洲淡水中对金属实施基于生物利用度的EQS。我们证明了完整的BLM预测与Bio-met预测的金属，铜，镍和锌的阈值（危险浓度影响5％物种[HC5]值的5％）之间的关系的可靠性。第一，将天然水实验室测试中特定物种的生态毒性数据与完整BLM中包含的单个物种BLM的预测进行了比较。其次，将由biomet预测的用于生态毒性测试的天然水的特定于现场的HC5值与完整的BLM提供的HC5值进行比较。证明了所有三种金属的两种关系的可靠性，其中超过80％的单个物种BLM预测值在实验结果的3倍之内，而99％的biometmet本地HC5预测值在2倍之内完整的BLM结果。第三，此外，使用更多的欧洲天然水集还证明了Bio-met在广泛的水化学条件下的可靠性。Environ Toxicol Chem 2020； 39：2361–2377。©2020 SETAC