Assessment of the in vivo biofunctionality of a biomimetic hybrid scaffold for osteochondral tissue regeneration,Biotechnology and Bioengineering

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Assessment of the in vivo biofunctionality of a biomimetic hybrid scaffold for osteochondral tissue regeneration
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-09-30 , DOI: 10.1002/bit.27584
Matilde Tschon ₁ , Silvia Brogini ₁ , Annapaola Parrilli ₂ , Serena Bertoldi _{3,

4} , Antonietta Silini ₅ , Ornella Parolini _{6,

6} , Silvia Faré _{3,

4} , Lucia Martini ₁ , Francesca Veronesi ₁ , Milena Fini ₁ , Gianluca Giavaresi ₁

Affiliation

Chondral and osteochondral lesions represent one of the most challenging problems in the orthopedic field, as these types of injuries lead to disability and worsened quality of life for patients and have an economic impact on the healthcare system. The aim of this in vivo study was to develop a new tissue engineering approach through a hybrid scaffold for osteochondral tissue regeneration made of porous polyurethane foam (PU) coated under vacuum with calcium phosphates (PU/VAC). Scaffold characterization showed a highly porous and interconnected structure. Human amniotic mesenchymal stromal cells (hAMSCs) were loaded into scaffolds using pectin (PECT) as a carrier. Osteochondral defects in medial femoral condyles of rabbits were created and randomly allocated in one of the following groups: plain scaffold (PU/VAC), scaffold with hAMSCs injected in the implant site (PU/VAC/hAMSC), scaffold with hAMSCs loaded in pectin (PU/VAC/PECT/hAMSC), and no treated defects (untreated). The therapeutic efficacy was assessed by macroscopic, histological, histomorphometric, microtomographic, and ultrastructural analyses at 3, 6, 12, and 24 weeks. Histological results showed that the scaffold was permissive to tissue growth and penetration, an immature osteocartilaginous tissue was observed at early experimental times, with a more accentuated bone regeneration in comparison with the cartilage layer in the absence of any inflammatory reaction.

中文翻译：

评估用于骨软骨组织再生的仿生混合支架的体内生物功能性

软骨和骨软骨病变是骨科领域最具挑战性的问题之一，因为这些类型的损伤会导致患者残疾和生活质量下降，并对医疗保健系统产生经济影响。这项体内研究的目的是通过混合支架开发一种新的组织工程方法，用于骨软骨组织再生，该支架由在真空下涂有磷酸钙 (PU/VAC) 的多孔聚氨酯泡沫 (PU) 制成。支架表征显示出高度多孔且相互连接的结构。使用果胶 (PECT) 作为载体将人羊膜间充质基质细胞 (hAMSCs) 加载到支架中。在兔子的股骨内侧髁中产生骨软骨缺损并随机分配到以下组之一：普通支架（PU/VAC），植入部位注射 hAMSCs 的支架 (PU/VAC/hAMSC)，载有果胶的 hAMSCs 支架 (PU/VAC/PECT/hAMSC)，无处理缺陷（未处理）。在第 3、6、12 和 24 周时通过宏观、组织学、组织形态计量学、显微断层扫描和超微结构分析评估治疗效果。组织学结果表明，支架允许组织生长和渗透，在早期实验时间观察到未成熟的骨软骨组织，与没有任何炎症反应的软骨层相比，骨再生更加明显。在第 3、6、12 和 24 周进行超微结构分析。组织学结果表明，支架允许组织生长和渗透，在早期实验时间观察到未成熟的骨软骨组织，与没有任何炎症反应的软骨层相比，骨再生更加明显。在第 3、6、12 和 24 周进行超微结构分析。组织学结果表明，支架允许组织生长和渗透，在早期实验时间观察到未成熟的骨软骨组织，与没有任何炎症反应的软骨层相比，骨再生更加明显。

更新日期：2020-09-30

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