Soil is an integral part of ecosystem which is niche for varieties of microflora. The present study was investigated to isolate varied strains of bacteria from soil samples of three different geographical regions of Tamil Nadu (India) and evaluate their hydrolytic enzymes (amylase, cellulase, and inulinase) producing potentialities. Among 72 bacterial cultures isolated from Ambattur Industrial Estate, Neyveli Lignite Corporation, and Arignar Anna Zoological Park regions, 41.66, 38.88, and 36.11% of isolates were observed amylase, cellulase, and inulinase producers, respectively. On the other hand, 20.83% of total bacteria isolated from all three regions exhibited concurrent production of amylase, cellulase, and inulinase. Potent isolates depicting maximum enzyme activities were identified as Bacillus anthracis strain ALA1, Bacillus cereus strain ALA3, Glutamicibacter arilaitensis strain ALA4, and Bacillus thuringiensis strain ALA5 based on molecular characterization tools. Further, the thermodynamics parameters, open reading frames (ORFs) regions, and guanine-cytosine (GC) content were determined by distinct bioinformatics tools using 16S rRNA sequences of strains. Minimum free energy values for strain ALA1, strain ALA3, strain ALA4, and strain ALA5 were calculated as −480.73, −478.76, −496.63, and −479.03 kcal/mol, respectively. Mountain plot and entropy predicted the hierarchical representation of RNA secondary structure. The GC content of sequence for strain ALA1, strain ALA3, strain ALA4, and strain ALA5 was calculated as 53.06, 52.94, 56.78, and 53.06%, respectively. Nine ORFs were obtained for strain ALA1, strain ALA3, and strain ALA5 while 10 ORFs were observed for strain ALA4. Additionally, bootstrap tree demonstrated close resemblance of strains with existing bacteria of similar genus. Findings showed higher variability of bacterial diversity as hydrolytic enzymes producers in the investigated geographical regions.

土壤是生态系统中不可或缺的一部分，对微生物群落的多样性具有独特的利基。本研究旨在从泰米尔纳德邦（印度）三个不同地理区域的土壤样品中分离出各种细菌，并评估其水解酶（淀粉酶，纤维素酶和菊粉酶）的生产潜力。从Ambattur工业区，Neyveli褐煤公司和Arignar Anna动物学园地区分离出的72种细菌培养物中，分别观察到41.66％，38.88％和36.11％的分离物是淀粉酶，纤维素酶和菊粉酶生产者。另一方面，从所有三个区域分离出的总细菌中有20.83％表现出同时产生淀粉酶，纤维素酶和菊粉酶。描绘最大酶活性的有效分离株被鉴定为炭疽芽孢杆菌基于分子鉴定工具，菌株ALA1，蜡样芽孢杆菌（Bacillus cereus）菌株ALA3，阿里氏Glutamicibacter arilaitensis菌株ALA4和苏云金芽孢杆菌（Bacillus thuringiensis）菌株ALA5。此外，通过独特的生物信息学工具，使用菌株的16S rRNA序列确定了热力学参数，开放阅读框（ORF）区域和鸟嘌呤-胞嘧啶（GC）含量。应变ALA1，应变ALA3，应变ALA4和应变ALA5的最小自由能值计算为-480.73，-分别为478.76，-496.63和-479.03 kcal / mol。山地图和熵预测了RNA二级结构的分层表示。计算出菌株ALA1，菌株ALA3，菌株ALA4和菌株ALA5的序列的GC含量分别为53.06、52.94、56.78和53.06％。对于菌株ALA1，菌株ALA3和菌株ALA5，获得了9个ORF，而对于菌株ALA4，则观察到了10个ORF。另外，自举树证明了菌株与具有相似属的现有细菌非常相似。调查结果显示，在所调查的地理区域中，作为水解酶生产商的细菌多样性具有较高的变异性。