Background

Spinal cord injury (SCI) is a serious central nervous system condition with no effective clinal treatment. Recently, transplantation of bone marrow mesenchymal stem cells (MSCs) derived exosomes has been proposed as a potential treatment for SCI. However, whether exosomes have similar functions as transplanted human placenta-derived MSCs(hPMSCs) has remained unclear.

Methods

The hPMSCs-derived exosomes (hPMSCs-Exos) were extracted using a sequential centrifugation approach. Then, the effects of hPMSCs-Exos on angiogenesis were analysis both in vitro and in vivo. In addition, the sensory and locomotor functions of mice after SCI were also analyzed.

Results

The administration of hPMSCs-Exos promote the tube formation and migration of human umbilical vein endothelial cell (HUVECs). Furthermore, vessel numbers, vessel volume fraction and vessel connectivity in spinal cords significantly increased after exosomes were intrathecally injected in the SCI model. In addition, the locomotor and sensory function, also significantly improved in the exosome treatment group.

Conclusions

The results of the present study demonstrated that hPMSCs-Exos have proangiogenic effects on endothelial cells and enhanced angiogenesis in SCI model. Thus, this treatment strategy demonstrates great potential for the treatment of SCI.

中文翻译：

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源自人胎盘的间充质干细胞衍生的外来体通过促进脊髓损伤后的血管生成来改善神经功能

背景

脊髓损伤（SCI）是严重的中枢神经系统疾病，没有有效的临床治疗。近来，已经提出了将骨髓间充质干细胞（MSC）衍生的外来体的移植作为SCI的潜在治疗方法。然而，外泌体是否具有与人类胎盘来源的MSC（hPMSCs）相似的功能。

方法

使用顺序离心方法提取hPMSCs衍生的外来体（hPMSCs-Exos）。然后，在体外和体内分析hPMSCs-Exos对血管生成的影响。另外，还分析了SCI后小鼠的感觉和运动功能。

结果

hPMSCs-Exos的给药促进人脐静脉内皮细胞（HUVECs）的管形成和迁移。此外，在SCI模型鞘内注射囊泡后，脊髓中的血管数目，血管体积分数和血管连通性显着增加。此外，外泌体治疗组的运动和感觉功能也明显改善。

结论

本研究结果表明，hPMSCs-Exos对SCI模型的内皮细胞具有促血管生成作用，并能增强血管生成。因此，该治疗策略证明了治疗SCI的巨大潜力。