当前位置: X-MOL 学术Int. J. Med. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates
International Journal of Medical Microbiology ( IF 4.5 ) Pub Date : 2020-09-29 , DOI: 10.1016/j.ijmm.2020.151454
Katerina Snopkova , Kristyna Dufkova , Petra Klimesova , Martina Vanerkova , Filip Ruzicka , Veronika Hola

Urinary tract infections represent common nosocomial infectious diseases. Bacteriocin production has been recently described as a putative virulence factor in these infections but studies focusing particularly on Pseudomonas aeruginosa are not available. Therefore, we assessed the prevalence of the bacteriocin genes, their co-occurrence and their co-association with previously detected virulence factors in a set of 135 P. aeruginosa strains from catheter-associated urinary tract infections (CAUTIs). The overall bacteriocinogeny reached 96.3 % with an average of 3.6 genes per strain. The most frequently detected determinants were the encoded pyocins S4 (76.3 %), R (69.6 %), and S2 (67.4 %). A statistically significant co-occurrence and a negative relationship were observed between several pyocin types. Particular pyocins exhibited associations with biofilm formation, production of pyochelin, pyocyanin, antibiotic-degrading enzymes, overall strain susceptibility and resistance, and motility of the strain. Co-occurrence of the pyocins S2 and S4 (p<<0.0001; Z = 13.15), both utilizating the ferripyoverdine receptor FpvAI, was found but no relation to pyoverdine production was detected. A negative association (p = 0.0047; Z=-2.83) was observed between pyochelin and pyocin S5 utilising the ferripyochelin receptor FptA. Pairwise assays resulted in 52.1 % inhibition which was equally distributed between soluble and particle types of antimicrobials. In conclusion, pyocin determinants appear to be important characteristics of CAUTI-related P. aeruginosa isolates and could contribute to their urovirulence.



中文翻译:

铜绿假单胞菌导管分离物中细菌素的流行及其与毒力因子的关联

尿路感染代表常见的医院传染病。近来,细菌素的生产已被描述为这些感染中的假定毒力因子,但尚无专门针对铜绿假单胞菌的研究。因此,我们评估了135种铜绿假单胞菌中细菌素基因的普遍性,它们的共现性以及它们与先前检测到的毒力因子的关联性导管相关性尿路感染(CAUTIs)引起的毒株。总体细菌致癌性达到96.3%,每个菌株平均3.6个基因。最常检测到的决定因素是编码的胞浆球蛋白S4(76.3%),R(69.6%)和S2(67.4%)。在几种脓毒症类型之间观察到统计学上显着的同时发生,并且具有负相关关系。特定的球藻蛋白酶表现出与生物膜的形成,pychelin,pyocyanin的产生,抗生素降解酶,整体菌株的敏感性和耐药性以及菌株的运动性相关。发现均利用铁氧嘧啶过氧化物受体FpvAI的胞嘧啶S2和S4共存(p << 0.0001; Z = 13.15),但未检测到与嘧啶的产生有关。负关联(p = 0.0047; Z = -2。利用铁磷脂酰肌醇蛋白受体FptA观察到了在卵磷脂和脓素S5之间的83)。配对分析产生52.1%的抑制作用,该抑制作用平均分布在可溶性和颗粒型抗菌剂之间。总之,黄霉素决定簇似乎是与CAUTI相关的重要特征铜绿假单胞菌分离株,可能有助于其尿毒力。

更新日期:2020-10-14
down
wechat
bug