In this study, the compound antimicrobial peptides was added to the basic diet to probe its effects on grass carp for 8 weeks. There were 6 groups in our study, including M0 (0 mg/kg) and 5 additional groups: M1 (100 mg/kg), M2 (200 mg/kg), M3 (400 mg/kg), M4 (800 mg/kg) and M5 (1600 mg/kg). The results are as follows: (1) The grass carp's FBW, WGR and SGR in M4 were significantly increased than M0 (p < 0.05). (2) In the hepatopancreas, the amylase activity of M2, M3 and M5 were significant up-regulation than other groups (p < 0.05). (3) The activity of T-AOC in the M3 and M4 was significantly higher compared to the other groups in grass carp hepatopancreas, while MDA was significantly reduced compared to the control group (p < 0.05). The activity of SOD in M5 was significantly increased than the other groups (p < 0.05). (4) The expression of MnSOD (except head kidney), IL8 and TNF-α and IL-1β (except hepatopancreas) were significantly increased (p < 0.05), while TGF-β and were significantly reduced in the hepatopancreas, spleen and head kidney at M3 (p < 0.05), and IL10 were significantly decreased in the hepatopancreas at M3 (p < 0.05). In addition, expression of IL8 and TNF-α were significant down-regulation, whereas TGF-β (expect head kidney) were significant up-regulation in hepatopancreas, spleen and head kidney in M3 after challenge with A. hydrophila. The expression of IL-1β in spleen and head kidney were also down-regulated, whereas IL10 were significantly up-regulated in the hepatopancreas at M3 after challenge with A. hydrophila (p < 0.05). The results of this study showed that grass carp fed a diet supplemented compound antimicrobial peptides was added with 400–800 mg/kg, which could improve the growth performance, antioxidant and digestive capabilities, and could also enhance the expression of immune-related genes and control to A. hydrophila.

在这项研究中，将复合抗菌肽添加到基本饮食中以探测其对草鱼的影响，持续8周。我们的研究分为6组，包括M0（0 mg / kg）和另外5组：M1（100 mg / kg），M2（200 mg / kg），M3（400 mg / kg），M4（800 mg / kg） kg）和M5（1600 mg / kg）。结果如下：（1）M4的草鱼的FBW，WGR和SGR均比M0显着增加（p  <0.05）。（2）在肝胰腺中，M2，M3和M5的淀粉酶活性明显高于其他组（p  <0.05）。（3）在草鱼肝胰腺中，M3和M4中的T-AOC活性明显高于其他组，而MDA则比对照组显着降低（p <0.05）。M5中的SOD活性明显高于其他组（p  <0.05）。（4）肝胰脏，脾脏和头部中MnSOD（除肾肾），IL8，TNF-α和IL-1β（除肝胰腺）的表达均显着升高（p  <0.05），而TGF-β则显着降低在M3时，肝胰腺中的肾脏（p  <0.05）和IL10显着降低（p  <0.05）。此外，IL8和TNF-α的表达明显下调，而TGF-β（预计头肾）均显著上调肝胰脏，脾脏和头肾在M3与挑战后，嗜水气单。亲水性链球菌攻击后，M3肝胰脏中脾脏和头肾中IL-1β的表达也下调，而IL10明显上调（p  <0.05）。研究结果表明，饲喂日粮添加复合抗菌肽的草鱼，添加量为400–800 mg / kg，可以改善生长性能，抗氧化和消化能力，还可以增强免疫相关基因的表达。防治嗜水杆菌。