Isoparvifuran is a benzofuran compound isolated from the heartwood of Dalbergia odorifera. Related research reported that isoparvifuran has antioxidant property. However, it is unclear whether isoparvifuran has anti-aging effects. In this research, we established an aging model, hydrogen peroxide (H₂O₂)-induced BJ cell senescence, to explore the protective effect of isoparvifuran on cell senescence and its related mechanisms. Our results revealed that isoparvifuran obviously attenuated H₂O₂-induced cell senescence, increased the cell proliferation rate,and reversed senescence-associated molecular markers expression such as cyclin D1, pRb, caveolin-1, ace-p53, p21 and p16. Moreover, isoparvifuran dose and time dependently increased the expression level of Sirtuin 1 (SIRT1) in BJ cells. The inhibition of SIRT1 obviously reversed the reduction of SA-β-gal activity and the alteration of senescence-associated molecular markers induced by isoparvifuran. Additionally, isoparvifuran also inhibited H₂O₂-induced AKT and S6 phosphorylation and increase of SA-β-gal activity. In summary, isoparvifuran protects BJ cells from H₂O₂-induced premature senescence, the anti-senescence effect of isoparvifuran is associated with the activation of SIRT1 and the suppression of AKT/mTOR signaling pathway.

异戊呋喃是一种从香檀香中分离的苯并呋喃化合物。相关研究报道，异帕维呋喃具有抗氧化性能。但是，尚不清楚异帕呋喃是否具有抗衰老作用。在本研究中，我们建立了一个过氧化氢（H ₂ O ₂）诱导的BJ细胞衰老的衰老模型，以探讨异帕维呋喃对细胞衰老的保护作用及其相关机制。我们的结果表明，异帕维呋喃明显减弱了H ₂ O ₂诱导的细胞衰老，增加细胞增殖速率，并逆转衰老相关分子标志物的表达，如细胞周期蛋白D1，pRb，caveolin-1，ace-p53，p21和p16。此外，异帕维呋喃的剂量和时间依赖性地增加了BJ细胞中Sirtuin 1（SIRT1）的表达水平。SIRT1的抑制作用明显逆转了异帕维呋喃诱导的SA-β-gal活性的降低和衰老相关分子标记的改变。此外，异帕维呋喃还抑制H ₂ O ₂诱导的AKT和S6磷酸化，并抑制SA-β-gal活性。总之，异帕维呋喃保护BJ细胞免受H ₂ O ₂侵害诱导的早衰，异帕维呋喃的抗衰老作用与SIRT1的激活和AKT / mTOR信号通路的抑制有关。