Algae extracts or clay are supplement into animal feed to promote growth, digestive performance and health status. A feeding trial was conducted to evaluate supplemental effects of an Algae-clay powder (sea lettuce, Ulva lactuca and red algae, Solieria chordalis in exfoliated micronized montmorillonite, ACP) into a low fish meal (LFM) diet on growth and feed utilization, digestibility, innate immunity and disease resistance of juvenile olive flounder (26.5 ± 0.02 g). A high fish meal diet (HFM) was prepared to contain 55 % FM and the LFM was prepared to contain 44 % FM. The ACP was added into the LFM diet by 0.2 % (MF2). Negative effects of LFM diet were observed in growth, feed utilization and diet digestibility of fish after 12 weeks of the feeding trial. The ACP inclusion in the LFM diet had positive effects on all parameters showing similar or higher growth performance, feed utilization, digestibility, goblet cell counts and villi lengths than the HFM diet. Non-specific immune response and antioxidant parameters, such as lysozyme, total immunoglobulin, anti-proteases, superoxide dismutase and catalase were also significantly improved by the addition of ACP into the LFM diet. Activities of digestive enzymes were improved by ACP supplementation compared to LFM group. Following the feeding trial, a 21-day long challenge test against Edwardsiella tarda showed that MF2 group had higher cumulative survival compared to that of HFM or LMF group while a decreased survival was found in the LFM group. The findings in this study indicate that ACP supplementation in a LFM diet can improve growth performance, feed utilization efficiency, digestibility with digestive enzyme activities, intestinal development, innate immunity and disease resistance of olive flounder.

藻类提取物或粘土是动物饲料的补充，以促进生长，消化性能和健康状况。进行了喂养试验，以评估藻类粘土粉（海生菜，Ulva lactuca和红藻，Solieria chordalis）的补充作用将脱落的微粉化蒙脱土（ACP）放入低鱼粉（LFM）饲料中，对少年比目鱼（26.5±0.02 g）的生长和饲料利用率，消化率，先天免疫力和抗病能力。高鱼粉饮食（HFM）制备为包含55％FM，LFM被制备为包含44％FM。ACP以0.2％（MF2）添加到LFM饮食中。在饲喂试验12周后，观察到LFM饮食对鱼类的生长，饲料利用率和饮食消化率具有负面影响。LFM日粮中的ACP对所有参数均具有积极作用，显示出与HFM日粮相似或更高的生长性能，饲料利用率，消化率，杯状细胞数和绒毛长度。非特异性免疫反应和抗氧化剂参数，例如溶菌酶，总免疫球蛋白，抗蛋白酶，通过在LFM饮食中添加ACP，也可以显着改善超氧化物歧化酶和过氧化氢酶。与LFM组相比，补充ACP可改善消化酶的活性。喂养试验后，进行了为期21天的挑战测试泰德华氏菌表明，MF2组比HFM或LMF组具有更高的累积存活率，而LFM组则具有较低的存活率。这项研究的发现表明，在LFM饮食中补充ACP可以改善橄榄比目鱼的生长性能，饲料利用效率，消化酶活性的消化率，肠道发育，先天免疫力和抗病性。