Algae extracts or clay are supplement into animal feed to promote growth, digestive performance and health status. A feeding trial was conducted to evaluate supplemental effects of an Algae-clay powder (sea lettuce, and red algae, in exfoliated micronized montmorillonite, ACP) into a low fish meal (LFM) diet on growth and feed utilization, digestibility, innate immunity and disease resistance of juvenile olive flounder (26.5 ± 0.02 g). A high fish meal diet (HFM) was prepared to contain 55 % FM and the LFM was prepared to contain 44 % FM. The ACP was added into the LFM diet by 0.2 % (MF2). Negative effects of LFM diet were observed in growth, feed utilization and diet digestibility of fish after 12 weeks of the feeding trial. The ACP inclusion in the LFM diet had positive effects on all parameters showing similar or higher growth performance, feed utilization, digestibility, goblet cell counts and villi lengths than the HFM diet. Non-specific immune response and antioxidant parameters, such as lysozyme, total immunoglobulin, anti-proteases, superoxide dismutase and catalase were also significantly improved by the addition of ACP into the LFM diet. Activities of digestive enzymes were improved by ACP supplementation compared to LFM group. Following the feeding trial, a 21-day long challenge test against showed that MF2 group had higher cumulative survival compared to that of HFM or LMF group while a decreased survival was found in the LFM group. The findings in this study indicate that ACP supplementation in a LFM diet can improve growth performance, feed utilization efficiency, digestibility with digestive enzyme activities, intestinal development, innate immunity and disease resistance of olive flounder.

中文翻译：

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藻类粘土粉（海生菜、石莼和红藻、剥落微粉化蒙脱石中的 Solieria cordalis）在橄榄鲽（Paralichthys olivaceus）的鱼粉减少饲料中添加


藻类提取物或粘土被添加到动物饲料中，以促进生长、消化性能和健康状况。进行了一项饲喂试验，以评估在低鱼粉 (LFM) 日粮中添加藻类粘土粉（海生菜和红藻，在剥离微粉化蒙脱石，ACP 中）对生长和饲料利用率、消化率、先天免疫力和橄榄鲽幼鱼的抗病性（26.5 ± 0.02 克）。高鱼粉日粮 (HFM) 中含有 55% FM，LFM 中含有 44% FM。 ACP 按 0.2% 添加到 LFM 日粮中 (M​​F2)。饲养试验 12 周后，观察到 LFM 日粮对鱼的生长、饲料利用率和日粮消化率产生负面影响。 LFM 日粮中添加 ACP 对所有参数均产生积极影响，显示出与 HFM 日粮相似或更高的生长性能、饲料利用率、消化率、杯状细胞计数和绒毛长度。在 LFM 日粮中添加 ACP 后，非特异性免疫反应和抗氧化参数，如溶菌酶、总免疫球蛋白、抗蛋白酶、超氧化物歧化酶和过氧化氢酶也得到显着改善。与 LFM 组相比，补充 ACP 后消化酶的活性得到改善。喂养试验后，为期21天的攻击测试表明，与HFM或LMF组相比，MF2组的累积生存率较高，而LFM组的生存率较低。本研究的结果表明，在 LFM 日粮中添加 ACP 可以提高橄榄鲽的生长性能、饲料利用率、消化酶活性的消化率、肠道发育、先天免疫和抗病能力。