Key message

ABCE model genes along with genes related to GA biosynthesis and auxin signalling may play significant roles in male flower development in Jatropha curcas.

Abstract

Flowering plants exhibit extreme reproductive diversity. Jatropha curcas, a woody plant that is promising for biofuel production, is monoecious. Here, two gynoecious Jatropha mutants (bearing only female flowers) were used to identify key genes involved in male flower development. Using comparative transcriptome analysis, we identified 17 differentially expressed genes (DEGs) involved in floral organ development between monoecious plants and the two gynoecious mutants. Among these DEGs, five floral organ identity genes, Jatropha AGAMOUS, PISTILLATA, SEPALLATA 2-1 (JcSEP2-1), JcSEP2-2, and JcSEP3, were downregulated in ch mutant inflorescences; two gibberellin (GA) biosynthesis genes, Jatropha GA REQUIRING 1 and GIBBERELLIN 3-OXIDASE 1, were downregulated in both the ch and g mutants; and two genes involved in the auxin signalling pathway, Jatropha NGATHA1 and STYLISH1, were downregulated in the ch mutant. Furthermore, four hub genes involved in male flower development, namely Jatropha SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1, CRYPTOCHROME 2, SUPPRESSOR OF OVEREXPRESSION OF CO 1 and JAGGED, were identified using weighted gene correlation network analysis. These results suggest that floral organ identity genes and genes involved in GA biosynthesis and auxin signalling may participate in male flower development in Jatropha. This study will contribute to understanding sex differentiation in woody perennial plants.

中文翻译：

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雌雄同株和雌雄同株花序的转录组比较分析表明，多年生木质植物麻风树麻疯树中参与雄花发育的调节剂

关键信息

ABCE模型基因以及与GA生物合成和生长素信号传导相关的基因可能在 麻疯树的雄花发育中发挥重要作用。

抽象

开花植物表现出极高的繁殖多样性。麻风树（Jatropha curcas）是一种有希望用于生物燃料生产的木本植物。在这里，使用了两个雌雄同株的麻风树突变体（仅携带雌花）来鉴定参与雄花发育的关键基因。使用比较转录组分析，我们确定了17种差异表达基因（DEG），它们参与了雌雄同株植物和两个雌雄同体突变体之间的花器官发育。在这些DEGS，五倍花器官身份的基因，麻疯AGAMOUS，PISTILLATA，SEPALLATA 2 - 1（JcSEP2 - 1），JcSEP2 - 2和JcSEP3在ch突变体的花序中被下调；ch和g突变体均下调了两个赤霉素（GA）生物合成基因，Jatropha GA REQUIRING 1和GIBBERELLIN 3 - OXIDASE 1。在ch突变体中，与生长素信号通路有关的两个基因Jatropha NGATHA1和STYLISH1被下调。此外，四个毂基因参与雄花发展，即麻风树体细胞胚发生受体-样激酶1，隐花色素2，使用加权基因相关网络分析确定了CO 1过表达的抑制子和杂物。这些结果表明，花器官身份基因和参与GA生物合成和生长素信号传导的基因可能参与了麻疯树的雄花发育。这项研究将有助于了解多年生木本植物的性别分化。